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Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues
BACKGROUND: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, mainly due to its high rates of postoperative recurrence and metastasis. Please remove, it currently ranks as the third most common cause of cancer-related deaths. MiRNAs are a set of small, single-stran...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528397/ https://www.ncbi.nlm.nih.gov/pubmed/26248880 http://dx.doi.org/10.1186/s13000-015-0371-4 |
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author | Ajdarkosh, Hossein Dadpay, Masoomeh Yahaghi, Emad Pirzaman, Elham Rostami Fayyaz, Amir Farshid Darian, Ebrahim Khodaverdi Mokarizadeh, Aram |
author_facet | Ajdarkosh, Hossein Dadpay, Masoomeh Yahaghi, Emad Pirzaman, Elham Rostami Fayyaz, Amir Farshid Darian, Ebrahim Khodaverdi Mokarizadeh, Aram |
author_sort | Ajdarkosh, Hossein |
collection | PubMed |
description | BACKGROUND: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, mainly due to its high rates of postoperative recurrence and metastasis. Please remove, it currently ranks as the third most common cause of cancer-related deaths. MiRNAs are a set of small, single-stranded, non-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level. In this study, we demonstrated the down-regulation of miR-148a in HCC and non-cancerous tissues using qRT-PCR. METHODS: Ninety six HCC samples and their noncancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-148a expression was determined using qRT-PCR. Furthermore, the correlation between the miR-148a expression and clinicopathological parameters was investigated. RESULTS: The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes. Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC in the current study. Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues. CONCLUSION: Down-regulation of miR-148a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients. |
format | Online Article Text |
id | pubmed-4528397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45283972015-08-08 Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues Ajdarkosh, Hossein Dadpay, Masoomeh Yahaghi, Emad Pirzaman, Elham Rostami Fayyaz, Amir Farshid Darian, Ebrahim Khodaverdi Mokarizadeh, Aram Diagn Pathol Research BACKGROUND: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, mainly due to its high rates of postoperative recurrence and metastasis. Please remove, it currently ranks as the third most common cause of cancer-related deaths. MiRNAs are a set of small, single-stranded, non-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level. In this study, we demonstrated the down-regulation of miR-148a in HCC and non-cancerous tissues using qRT-PCR. METHODS: Ninety six HCC samples and their noncancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-148a expression was determined using qRT-PCR. Furthermore, the correlation between the miR-148a expression and clinicopathological parameters was investigated. RESULTS: The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes. Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC in the current study. Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues. CONCLUSION: Down-regulation of miR-148a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients. BioMed Central 2015-08-07 /pmc/articles/PMC4528397/ /pubmed/26248880 http://dx.doi.org/10.1186/s13000-015-0371-4 Text en © Ajdarkosh et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ajdarkosh, Hossein Dadpay, Masoomeh Yahaghi, Emad Pirzaman, Elham Rostami Fayyaz, Amir Farshid Darian, Ebrahim Khodaverdi Mokarizadeh, Aram Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues |
title | Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues |
title_full | Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues |
title_fullStr | Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues |
title_full_unstemmed | Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues |
title_short | Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues |
title_sort | decrease expression and clinicopathological significance of mir-148a with poor survival in hepatocellular carcinoma tissues |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528397/ https://www.ncbi.nlm.nih.gov/pubmed/26248880 http://dx.doi.org/10.1186/s13000-015-0371-4 |
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