Cargando…

A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus

BACKGROUND: The aim of this study was to investigate the presence of circoviruses in wild bird populations, in Poland. Circoviruses possess immuno-suppressive properties and might interfere with the health of wild birds. METHOD: 83 birds, which belonged to 23 species, were tested with broad-range, n...

Descripción completa

Detalles Bibliográficos
Autores principales: Matczuk, Anna Karolina, Krawiec, Marta, Wieliczko, Alina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528844/
https://www.ncbi.nlm.nih.gov/pubmed/26253134
http://dx.doi.org/10.1186/s12985-015-0352-y
_version_ 1782384713659318272
author Matczuk, Anna Karolina
Krawiec, Marta
Wieliczko, Alina
author_facet Matczuk, Anna Karolina
Krawiec, Marta
Wieliczko, Alina
author_sort Matczuk, Anna Karolina
collection PubMed
description BACKGROUND: The aim of this study was to investigate the presence of circoviruses in wild bird populations, in Poland. Circoviruses possess immuno-suppressive properties and might interfere with the health of wild birds. METHOD: 83 birds, which belonged to 23 species, were tested with broad-range, nested PCR. The obtained PCR products were sequenced and new primers designed, to analyse the full-length, viral genome. A phylogenetic analysis was conducted, to find any relationship to known circoviruses. RESULTS: The circovirus DNA sequence was found in 4 birds. All samples originated from the velvet scoter (Melanitta fusca) a marine duck from the Merginae sub-family. Birds which tested positive for the circovirus were found dead in fishing nets, off the Baltic coast. During post-mortem examination, carcasses of two of the scoters showed only light emaciation, while the two other birds appeared healthy. The obtained, full-length, circovirus sequence revealed 1,988 nucleotides and the presence of typical features (i.e. Cap, Rep and ORF3). Nucleotide similarity to other duck circoviruses was 84 to 86 %. Phylogenetic analysis of the complete genome and cap gene, indicated that the new circovirus is related to known duck circoviruses, especially to sub-types sometimes referred to as duck circovirus genotype 1, but not genotype 2. CONCLUSIONS: In this study, we have reported a new duck circovirus sequence detected in the velvet scoter, a species of marine duck. Sequence comparison and phylogenetic analysis of the new virus sequence support previous reports that duck circovirus (DuCV) is a species with a high degree of diversity. The viral sequence obtained from the velvet scoter suggests that DuCV may infect birds from the Anatinae sub-family. More studies are needed to prove if the velvet scoter and other marine ducks act as a reservoir for DuCV.
format Online
Article
Text
id pubmed-4528844
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-45288442015-08-08 A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus Matczuk, Anna Karolina Krawiec, Marta Wieliczko, Alina Virol J Research BACKGROUND: The aim of this study was to investigate the presence of circoviruses in wild bird populations, in Poland. Circoviruses possess immuno-suppressive properties and might interfere with the health of wild birds. METHOD: 83 birds, which belonged to 23 species, were tested with broad-range, nested PCR. The obtained PCR products were sequenced and new primers designed, to analyse the full-length, viral genome. A phylogenetic analysis was conducted, to find any relationship to known circoviruses. RESULTS: The circovirus DNA sequence was found in 4 birds. All samples originated from the velvet scoter (Melanitta fusca) a marine duck from the Merginae sub-family. Birds which tested positive for the circovirus were found dead in fishing nets, off the Baltic coast. During post-mortem examination, carcasses of two of the scoters showed only light emaciation, while the two other birds appeared healthy. The obtained, full-length, circovirus sequence revealed 1,988 nucleotides and the presence of typical features (i.e. Cap, Rep and ORF3). Nucleotide similarity to other duck circoviruses was 84 to 86 %. Phylogenetic analysis of the complete genome and cap gene, indicated that the new circovirus is related to known duck circoviruses, especially to sub-types sometimes referred to as duck circovirus genotype 1, but not genotype 2. CONCLUSIONS: In this study, we have reported a new duck circovirus sequence detected in the velvet scoter, a species of marine duck. Sequence comparison and phylogenetic analysis of the new virus sequence support previous reports that duck circovirus (DuCV) is a species with a high degree of diversity. The viral sequence obtained from the velvet scoter suggests that DuCV may infect birds from the Anatinae sub-family. More studies are needed to prove if the velvet scoter and other marine ducks act as a reservoir for DuCV. BioMed Central 2015-08-08 /pmc/articles/PMC4528844/ /pubmed/26253134 http://dx.doi.org/10.1186/s12985-015-0352-y Text en © Matczuk et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Matczuk, Anna Karolina
Krawiec, Marta
Wieliczko, Alina
A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus
title A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus
title_full A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus
title_fullStr A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus
title_full_unstemmed A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus
title_short A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus
title_sort new duck circovirus sequence, detected in velvet scoter (melanitta fusca) supports great diversity among this species of virus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528844/
https://www.ncbi.nlm.nih.gov/pubmed/26253134
http://dx.doi.org/10.1186/s12985-015-0352-y
work_keys_str_mv AT matczukannakarolina anewduckcircovirussequencedetectedinvelvetscotermelanittafuscasupportsgreatdiversityamongthisspeciesofvirus
AT krawiecmarta anewduckcircovirussequencedetectedinvelvetscotermelanittafuscasupportsgreatdiversityamongthisspeciesofvirus
AT wieliczkoalina anewduckcircovirussequencedetectedinvelvetscotermelanittafuscasupportsgreatdiversityamongthisspeciesofvirus
AT matczukannakarolina newduckcircovirussequencedetectedinvelvetscotermelanittafuscasupportsgreatdiversityamongthisspeciesofvirus
AT krawiecmarta newduckcircovirussequencedetectedinvelvetscotermelanittafuscasupportsgreatdiversityamongthisspeciesofvirus
AT wieliczkoalina newduckcircovirussequencedetectedinvelvetscotermelanittafuscasupportsgreatdiversityamongthisspeciesofvirus