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The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma
Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis. The aim of the study was to determine the lncRNA expression profile in astrocytomas and to assess its potential clinical value. We performed a three-step analysis to establish the lncRNA profile for astro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529097/ https://www.ncbi.nlm.nih.gov/pubmed/26252651 http://dx.doi.org/10.1371/journal.pone.0135242 |
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author | Zhi, Feng Wang, Qiang Xue, Lian Shao, Naiyuan Wang, Rong Deng, Danni Wang, Suinuan Xia, Xiwei Yang, Yilin |
author_facet | Zhi, Feng Wang, Qiang Xue, Lian Shao, Naiyuan Wang, Rong Deng, Danni Wang, Suinuan Xia, Xiwei Yang, Yilin |
author_sort | Zhi, Feng |
collection | PubMed |
description | Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis. The aim of the study was to determine the lncRNA expression profile in astrocytomas and to assess its potential clinical value. We performed a three-step analysis to establish the lncRNA profile for astrocytoma: a) the lncRNA expression was examined on 3 astrocytomas as well as 3 NATs (normal adjacent tissues) using the lncRNA microarray; b) the top-hits were validated in 40 astrocytomas (WHO grade II-IV) by quantitative real time-PCR (qRT-PCR); c) the hits with significant differences were re-evaluated using qRT-PCR in 90 astrocytomas. Finally, 7 lncRNAs were found to have a significantly different expression profile in astrocytoma samples compared to the NAT samples. Unsupervised clustering analysis further revealed the potential of the 7-lncRNA profile to differentiate between tumors and NAT samples. The upregulation of ENST00000545440 and NR_002809 was associated with advanced clinical stages of astrocytoma. Using Kaplan-Meier survival analysis, we showed that the low expression of BC002811 or XLOC_010967, or the high expression of NR_002809 was significantly associated with poor patient survival. Moreover, Cox proportional hazard regression analysis revealed that this prognostic impact was independent of other clinicopathological factors. Our results indicate that the lncRNA profile may be a potential prognostic biomarker for the prediction of post-surgical outcomes. |
format | Online Article Text |
id | pubmed-4529097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45290972015-08-12 The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma Zhi, Feng Wang, Qiang Xue, Lian Shao, Naiyuan Wang, Rong Deng, Danni Wang, Suinuan Xia, Xiwei Yang, Yilin PLoS One Research Article Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis. The aim of the study was to determine the lncRNA expression profile in astrocytomas and to assess its potential clinical value. We performed a three-step analysis to establish the lncRNA profile for astrocytoma: a) the lncRNA expression was examined on 3 astrocytomas as well as 3 NATs (normal adjacent tissues) using the lncRNA microarray; b) the top-hits were validated in 40 astrocytomas (WHO grade II-IV) by quantitative real time-PCR (qRT-PCR); c) the hits with significant differences were re-evaluated using qRT-PCR in 90 astrocytomas. Finally, 7 lncRNAs were found to have a significantly different expression profile in astrocytoma samples compared to the NAT samples. Unsupervised clustering analysis further revealed the potential of the 7-lncRNA profile to differentiate between tumors and NAT samples. The upregulation of ENST00000545440 and NR_002809 was associated with advanced clinical stages of astrocytoma. Using Kaplan-Meier survival analysis, we showed that the low expression of BC002811 or XLOC_010967, or the high expression of NR_002809 was significantly associated with poor patient survival. Moreover, Cox proportional hazard regression analysis revealed that this prognostic impact was independent of other clinicopathological factors. Our results indicate that the lncRNA profile may be a potential prognostic biomarker for the prediction of post-surgical outcomes. Public Library of Science 2015-08-07 /pmc/articles/PMC4529097/ /pubmed/26252651 http://dx.doi.org/10.1371/journal.pone.0135242 Text en © 2015 Zhi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhi, Feng Wang, Qiang Xue, Lian Shao, Naiyuan Wang, Rong Deng, Danni Wang, Suinuan Xia, Xiwei Yang, Yilin The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma |
title | The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma |
title_full | The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma |
title_fullStr | The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma |
title_full_unstemmed | The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma |
title_short | The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma |
title_sort | use of three long non-coding rnas as potential prognostic indicators of astrocytoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529097/ https://www.ncbi.nlm.nih.gov/pubmed/26252651 http://dx.doi.org/10.1371/journal.pone.0135242 |
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