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Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent

The aim of this study was to determine the best processing conditions to extract Brazilian green propolis using a supercritical extraction technology. For this purpose, the influence of different parameters was evaluated such as S/F (solvent mass in relation to solute mass), percentage of co-solvent...

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Autores principales: Machado, Bruna Aparecida Souza, Barreto, Gabriele de Abreu, Costa, Aline Silva, Costa, Samantha Serra, Silva, Rejane Pina Dantas, da Silva, Danielle Figuerêdo, Brandão, Hugo Neves, da Rocha, José Luiz Carneiro, Nunes, Silmar Baptista, Umsza-Guez, Marcelo Andres, Padilha, Francine Ferreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529176/
https://www.ncbi.nlm.nih.gov/pubmed/26252491
http://dx.doi.org/10.1371/journal.pone.0134489
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author Machado, Bruna Aparecida Souza
Barreto, Gabriele de Abreu
Costa, Aline Silva
Costa, Samantha Serra
Silva, Rejane Pina Dantas
da Silva, Danielle Figuerêdo
Brandão, Hugo Neves
da Rocha, José Luiz Carneiro
Nunes, Silmar Baptista
Umsza-Guez, Marcelo Andres
Padilha, Francine Ferreira
author_facet Machado, Bruna Aparecida Souza
Barreto, Gabriele de Abreu
Costa, Aline Silva
Costa, Samantha Serra
Silva, Rejane Pina Dantas
da Silva, Danielle Figuerêdo
Brandão, Hugo Neves
da Rocha, José Luiz Carneiro
Nunes, Silmar Baptista
Umsza-Guez, Marcelo Andres
Padilha, Francine Ferreira
author_sort Machado, Bruna Aparecida Souza
collection PubMed
description The aim of this study was to determine the best processing conditions to extract Brazilian green propolis using a supercritical extraction technology. For this purpose, the influence of different parameters was evaluated such as S/F (solvent mass in relation to solute mass), percentage of co-solvent (1 and 2% ethanol), temperature (40 and 50°C) and pressure (250, 350 and 400 bar) using supercritical carbon dioxide. The Global Yield Isotherms (GYIs) were obtained through the evaluation of the yield, and the chemical composition of the extracts was also obtained in relation to the total phenolic compounds, flavonoids, antioxidant activity and 3,5-diprenyl-4-hydroxicinnamic acid (Artepillin C) and acid 4-hydroxycinnamic (p-coumaric acid). The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110. These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process. Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition.
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spelling pubmed-45291762015-08-12 Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent Machado, Bruna Aparecida Souza Barreto, Gabriele de Abreu Costa, Aline Silva Costa, Samantha Serra Silva, Rejane Pina Dantas da Silva, Danielle Figuerêdo Brandão, Hugo Neves da Rocha, José Luiz Carneiro Nunes, Silmar Baptista Umsza-Guez, Marcelo Andres Padilha, Francine Ferreira PLoS One Research Article The aim of this study was to determine the best processing conditions to extract Brazilian green propolis using a supercritical extraction technology. For this purpose, the influence of different parameters was evaluated such as S/F (solvent mass in relation to solute mass), percentage of co-solvent (1 and 2% ethanol), temperature (40 and 50°C) and pressure (250, 350 and 400 bar) using supercritical carbon dioxide. The Global Yield Isotherms (GYIs) were obtained through the evaluation of the yield, and the chemical composition of the extracts was also obtained in relation to the total phenolic compounds, flavonoids, antioxidant activity and 3,5-diprenyl-4-hydroxicinnamic acid (Artepillin C) and acid 4-hydroxycinnamic (p-coumaric acid). The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110. These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process. Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition. Public Library of Science 2015-08-07 /pmc/articles/PMC4529176/ /pubmed/26252491 http://dx.doi.org/10.1371/journal.pone.0134489 Text en © 2015 Machado et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Machado, Bruna Aparecida Souza
Barreto, Gabriele de Abreu
Costa, Aline Silva
Costa, Samantha Serra
Silva, Rejane Pina Dantas
da Silva, Danielle Figuerêdo
Brandão, Hugo Neves
da Rocha, José Luiz Carneiro
Nunes, Silmar Baptista
Umsza-Guez, Marcelo Andres
Padilha, Francine Ferreira
Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent
title Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent
title_full Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent
title_fullStr Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent
title_full_unstemmed Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent
title_short Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent
title_sort determination of parameters for the supercritical extraction of antioxidant compounds from green propolis using carbon dioxide and ethanol as co-solvent
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529176/
https://www.ncbi.nlm.nih.gov/pubmed/26252491
http://dx.doi.org/10.1371/journal.pone.0134489
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