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In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy

Metals, such as nickel, cobalt, chromium and zinc, are ubiquitous in the environment. Systemic reactions, including hand dermatitis and generalized eczematous reactions, can be caused by the dietary ingestion of metals. In this study, we aimed to determine whether the cytokine production from periph...

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Autores principales: Yoshihisa, Yoko, Rehman, Mati Ur, Yamakoshi-Shibutani, Takako, Shimizu, Tadamichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529423/
https://www.ncbi.nlm.nih.gov/pubmed/26261762
http://dx.doi.org/10.1186/s40064-015-1202-5
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author Yoshihisa, Yoko
Rehman, Mati Ur
Yamakoshi-Shibutani, Takako
Shimizu, Tadamichi
author_facet Yoshihisa, Yoko
Rehman, Mati Ur
Yamakoshi-Shibutani, Takako
Shimizu, Tadamichi
author_sort Yoshihisa, Yoko
collection PubMed
description Metals, such as nickel, cobalt, chromium and zinc, are ubiquitous in the environment. Systemic reactions, including hand dermatitis and generalized eczematous reactions, can be caused by the dietary ingestion of metals. In this study, we aimed to determine whether the cytokine production from peripheral blood mononuclear cells (PBMCs) obtained from zinc allergy patients can be used as a sensitive marker to investigate zinc-allergic contact dermatitis. The diagnosis of sensitivity to metal was made based on the results of a metal patch test. The PBMCs were stimulated with various concentrations (5–100 μM) of zinc sulfate (ZnSO(4)) for 24 h. The culture supernatants were collected and analyzed using ELISA for measurement of the cytokine production. The levels of IFN-γ, TNF-α, IL-1β, IL-5, IL-13 and MIF were significantly higher in the zinc-allergic patients (n = 5) than in the healthy controls (n = 5) at 100 μM of ZnSO(4) stimulation. Although, patch testing is considered as standard test to diagnose metal allergy but false-positive and -negative reactions may limit its use in conditions of existing dermatitis. Therefore, this study suggest that in support of patch testing the determination of cytokine production using PBMCs cultures would be helpful for making an early diagnosis of such conditions.
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spelling pubmed-45294232015-08-10 In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy Yoshihisa, Yoko Rehman, Mati Ur Yamakoshi-Shibutani, Takako Shimizu, Tadamichi Springerplus Research Metals, such as nickel, cobalt, chromium and zinc, are ubiquitous in the environment. Systemic reactions, including hand dermatitis and generalized eczematous reactions, can be caused by the dietary ingestion of metals. In this study, we aimed to determine whether the cytokine production from peripheral blood mononuclear cells (PBMCs) obtained from zinc allergy patients can be used as a sensitive marker to investigate zinc-allergic contact dermatitis. The diagnosis of sensitivity to metal was made based on the results of a metal patch test. The PBMCs were stimulated with various concentrations (5–100 μM) of zinc sulfate (ZnSO(4)) for 24 h. The culture supernatants were collected and analyzed using ELISA for measurement of the cytokine production. The levels of IFN-γ, TNF-α, IL-1β, IL-5, IL-13 and MIF were significantly higher in the zinc-allergic patients (n = 5) than in the healthy controls (n = 5) at 100 μM of ZnSO(4) stimulation. Although, patch testing is considered as standard test to diagnose metal allergy but false-positive and -negative reactions may limit its use in conditions of existing dermatitis. Therefore, this study suggest that in support of patch testing the determination of cytokine production using PBMCs cultures would be helpful for making an early diagnosis of such conditions. Springer International Publishing 2015-08-08 /pmc/articles/PMC4529423/ /pubmed/26261762 http://dx.doi.org/10.1186/s40064-015-1202-5 Text en © Yoshihisa et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Yoshihisa, Yoko
Rehman, Mati Ur
Yamakoshi-Shibutani, Takako
Shimizu, Tadamichi
In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy
title In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy
title_full In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy
title_fullStr In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy
title_full_unstemmed In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy
title_short In vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy
title_sort in vitro effects of zinc on the cytokine production from peripheral blood mononuclear cells in patients with zinc allergy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529423/
https://www.ncbi.nlm.nih.gov/pubmed/26261762
http://dx.doi.org/10.1186/s40064-015-1202-5
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