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Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder

BACKGROUND: Voiding dysfunctions are a common problem that has a severe negative impact on the quality of life. Today there is a need for new drug targets for these conditions. The role of ATP receptors in bladder physiology has been studied for some time, primarily in animal models. The aim of this...

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Autores principales: Svennersten, Karl, Hallén-Grufman, Katarina, de Verdier, Petra J., Wiklund, N. Peter, Poljakovic, Mirjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529706/
https://www.ncbi.nlm.nih.gov/pubmed/26253104
http://dx.doi.org/10.1186/s12894-015-0075-9
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author Svennersten, Karl
Hallén-Grufman, Katarina
de Verdier, Petra J.
Wiklund, N. Peter
Poljakovic, Mirjana
author_facet Svennersten, Karl
Hallén-Grufman, Katarina
de Verdier, Petra J.
Wiklund, N. Peter
Poljakovic, Mirjana
author_sort Svennersten, Karl
collection PubMed
description BACKGROUND: Voiding dysfunctions are a common problem that has a severe negative impact on the quality of life. Today there is a need for new drug targets for these conditions. The role of ATP receptors in bladder physiology has been studied for some time, primarily in animal models. The aim of this work is to investigate the localization of the ATP receptors P2X2, P2X3 and P2X7 and their colocalization with vimentin and actin in the human urinary bladder. METHODS: Immunohistochemical analysis was conducted on full-thickness bladder tissues from fundus and trigonum collected from 15 patients undergoing open radical cystectomy due to chronic cystitis, bladder cancer or locally advanced prostate cancer. Colocalization analyses were performed between the three different P2X subtypes and the structural proteins vimentin and actin. Specimens were examined using epifluorescence microscopy and correlation coefficients were calculated for each costaining as well as the mean distance from the laminin positive basal side of the urothelium to the vimentin positive cells located in the suburothelium. RESULTS: P2X2 was expressed in vimentin positive cells located in the suburothelium. Less distinct labelling of P2X2 was also observed in actin positive smooth muscle cells and in the urothelium. P2X3 was expressed in vimentin positive cells surrounding the smooth muscle, and in vimentin positive cells located in the suburothelium. Weaker P2X3 labelling was seen in the urothelium. P2X7 was expressed in the smooth muscle cells and the urothelium. In the suburothelium, cells double positive for P2X2 and vimentin where located closer to the urothelium while cells double positive for P2X3 and vimentin where located further from the urothelium. CONCLUSION: The results from this study demonstrate that there is a significant difference in the expression of the purinergic P2X2, P2X3 and P2X7 receptors in the different histological layers of the human urinary bladder.
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spelling pubmed-45297062015-08-09 Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder Svennersten, Karl Hallén-Grufman, Katarina de Verdier, Petra J. Wiklund, N. Peter Poljakovic, Mirjana BMC Urol Research Article BACKGROUND: Voiding dysfunctions are a common problem that has a severe negative impact on the quality of life. Today there is a need for new drug targets for these conditions. The role of ATP receptors in bladder physiology has been studied for some time, primarily in animal models. The aim of this work is to investigate the localization of the ATP receptors P2X2, P2X3 and P2X7 and their colocalization with vimentin and actin in the human urinary bladder. METHODS: Immunohistochemical analysis was conducted on full-thickness bladder tissues from fundus and trigonum collected from 15 patients undergoing open radical cystectomy due to chronic cystitis, bladder cancer or locally advanced prostate cancer. Colocalization analyses were performed between the three different P2X subtypes and the structural proteins vimentin and actin. Specimens were examined using epifluorescence microscopy and correlation coefficients were calculated for each costaining as well as the mean distance from the laminin positive basal side of the urothelium to the vimentin positive cells located in the suburothelium. RESULTS: P2X2 was expressed in vimentin positive cells located in the suburothelium. Less distinct labelling of P2X2 was also observed in actin positive smooth muscle cells and in the urothelium. P2X3 was expressed in vimentin positive cells surrounding the smooth muscle, and in vimentin positive cells located in the suburothelium. Weaker P2X3 labelling was seen in the urothelium. P2X7 was expressed in the smooth muscle cells and the urothelium. In the suburothelium, cells double positive for P2X2 and vimentin where located closer to the urothelium while cells double positive for P2X3 and vimentin where located further from the urothelium. CONCLUSION: The results from this study demonstrate that there is a significant difference in the expression of the purinergic P2X2, P2X3 and P2X7 receptors in the different histological layers of the human urinary bladder. BioMed Central 2015-08-08 /pmc/articles/PMC4529706/ /pubmed/26253104 http://dx.doi.org/10.1186/s12894-015-0075-9 Text en © Svennersten et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Svennersten, Karl
Hallén-Grufman, Katarina
de Verdier, Petra J.
Wiklund, N. Peter
Poljakovic, Mirjana
Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder
title Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder
title_full Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder
title_fullStr Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder
title_full_unstemmed Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder
title_short Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder
title_sort localization of p2x receptor subtypes 2, 3 and 7 in human urinary bladder
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529706/
https://www.ncbi.nlm.nih.gov/pubmed/26253104
http://dx.doi.org/10.1186/s12894-015-0075-9
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