Rapid detection of immunoglobulin heavy chain gene rearrangement by PCR and melting curve analysis using combined FR2 and FR3 primers

BACKGROUND: Immunoglobulin heavy chain (IgH) gene rearrangement test is a standard tool in diagnosing B-cell lymphoma. The BIOMED-2 multiplex PCR protocol has become the most commonly used laboratory method for detecting clonal IgH gene rearrangement. However, post-PCR procedure requires manual tran...

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Detalles Bibliográficos
Autores principales: Xu, Danfei, Yang, Zhuo, Zhang, Donghong, Wu, Wei, Guo, Ye, Chen, Qian, Xu, Dongsheng, Cui, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529721/
https://www.ncbi.nlm.nih.gov/pubmed/26255311
http://dx.doi.org/10.1186/s13000-015-0370-5

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