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Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis

Previous developmental studies of the thalamus (alar part of the diencephalic prosomere p2) have defined the molecular basis for the acquisition of the thalamic competence (preparttening), the subsequent formation of the secondary organizer in the zona limitans intrathalamica, and the early specific...

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Autores principales: Bandín, Sandra, Morona, Ruth, González, Agustín
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4530589/
https://www.ncbi.nlm.nih.gov/pubmed/26321920
http://dx.doi.org/10.3389/fnana.2015.00107
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author Bandín, Sandra
Morona, Ruth
González, Agustín
author_facet Bandín, Sandra
Morona, Ruth
González, Agustín
author_sort Bandín, Sandra
collection PubMed
description Previous developmental studies of the thalamus (alar part of the diencephalic prosomere p2) have defined the molecular basis for the acquisition of the thalamic competence (preparttening), the subsequent formation of the secondary organizer in the zona limitans intrathalamica, and the early specification of two anteroposterior domains (rostral and caudal progenitor domains) in response to inducing activities and that are shared in birds and mammals. In the present study we have analyzed the embryonic development of the thalamus in the anuran Xenopus laevis to determine conserved or specific features in the amphibian diencephalon. From early embryonic stages to the beginning of the larval period, the expression patterns of 22 markers were analyzed by means of combined In situ hybridization (ISH) and immunohistochemical techniques. The early genoarchitecture observed in the diencephalon allowed us to discern the boundaries of the thalamus with the prethalamus, pretectum, and epithalamus. Common molecular features were observed in the thalamic prepatterning among vertebrates in which Wnt3a, Fez, Pax6 and Xiro1 expression were of particular importance in Xenopus. The formation of the zona limitans intrathalamica was observed, as in other vertebrates, by the progressive expression of Shh. The largely conserved expressions of Nkx2.2 in the rostral thalamic domain vs. Gbx2 and Ngn2 (among others) in the caudal domain strongly suggest the role of Shh as morphogen in the amphibian thalamus. All these data showed that the molecular characteristics observed during preparttening and patterning in the thalamus of the anuran Xenopus (anamniote) share many features with those described during thalamic development in amniotes (common patterns in tetrapods) but also with zebrafish, strengthening the idea of a basic organization of this diencephalic region across vertebrates.
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spelling pubmed-45305892015-08-28 Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis Bandín, Sandra Morona, Ruth González, Agustín Front Neuroanat Neuroscience Previous developmental studies of the thalamus (alar part of the diencephalic prosomere p2) have defined the molecular basis for the acquisition of the thalamic competence (preparttening), the subsequent formation of the secondary organizer in the zona limitans intrathalamica, and the early specification of two anteroposterior domains (rostral and caudal progenitor domains) in response to inducing activities and that are shared in birds and mammals. In the present study we have analyzed the embryonic development of the thalamus in the anuran Xenopus laevis to determine conserved or specific features in the amphibian diencephalon. From early embryonic stages to the beginning of the larval period, the expression patterns of 22 markers were analyzed by means of combined In situ hybridization (ISH) and immunohistochemical techniques. The early genoarchitecture observed in the diencephalon allowed us to discern the boundaries of the thalamus with the prethalamus, pretectum, and epithalamus. Common molecular features were observed in the thalamic prepatterning among vertebrates in which Wnt3a, Fez, Pax6 and Xiro1 expression were of particular importance in Xenopus. The formation of the zona limitans intrathalamica was observed, as in other vertebrates, by the progressive expression of Shh. The largely conserved expressions of Nkx2.2 in the rostral thalamic domain vs. Gbx2 and Ngn2 (among others) in the caudal domain strongly suggest the role of Shh as morphogen in the amphibian thalamus. All these data showed that the molecular characteristics observed during preparttening and patterning in the thalamus of the anuran Xenopus (anamniote) share many features with those described during thalamic development in amniotes (common patterns in tetrapods) but also with zebrafish, strengthening the idea of a basic organization of this diencephalic region across vertebrates. Frontiers Media S.A. 2015-08-10 /pmc/articles/PMC4530589/ /pubmed/26321920 http://dx.doi.org/10.3389/fnana.2015.00107 Text en Copyright © 2015 Bandín, Morona and González. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Bandín, Sandra
Morona, Ruth
González, Agustín
Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis
title Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis
title_full Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis
title_fullStr Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis
title_full_unstemmed Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis
title_short Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis
title_sort prepatterning and patterning of the thalamus along embryonic development of xenopus laevis
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4530589/
https://www.ncbi.nlm.nih.gov/pubmed/26321920
http://dx.doi.org/10.3389/fnana.2015.00107
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