Cargando…

Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli

Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A...

Descripción completa

Detalles Bibliográficos
Autores principales: Parveez, Ghulam Kadir Ahmad, Bahariah, Bohari, Ayub, Nor Hanin, Masani, Mat Yunus Abdul, Rasid, Omar Abdul, Tarmizi, Ahmad Hashim, Ishak, Zamzuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531230/
https://www.ncbi.nlm.nih.gov/pubmed/26322053
http://dx.doi.org/10.3389/fpls.2015.00598
_version_ 1782385012073562112
author Parveez, Ghulam Kadir Ahmad
Bahariah, Bohari
Ayub, Nor Hanin
Masani, Mat Yunus Abdul
Rasid, Omar Abdul
Tarmizi, Ahmad Hashim
Ishak, Zamzuri
author_facet Parveez, Ghulam Kadir Ahmad
Bahariah, Bohari
Ayub, Nor Hanin
Masani, Mat Yunus Abdul
Rasid, Omar Abdul
Tarmizi, Ahmad Hashim
Ishak, Zamzuri
author_sort Parveez, Ghulam Kadir Ahmad
collection PubMed
description Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA), producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB, and phaC, which are required for the synthesis of PHB and selectable marker gene, bar, for herbicide Basta resistant, were transformed into embryogenic calli. A number of transformed embryogenic lines resistant to herbicide Basta were obtained and were later regenerated to produce few hundred plantlets. Molecular analyses, including polymerase chain reaction (PCR), Southern blot, and real-time PCR have demonstrated stable integration and expression of the transgenes in the oil palm genome. HPLC and Nile blue A staining analyses confirmed the synthesis of PHB in some of the plantlets.
format Online
Article
Text
id pubmed-4531230
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-45312302015-08-28 Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli Parveez, Ghulam Kadir Ahmad Bahariah, Bohari Ayub, Nor Hanin Masani, Mat Yunus Abdul Rasid, Omar Abdul Tarmizi, Ahmad Hashim Ishak, Zamzuri Front Plant Sci Plant Science Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA), producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB, and phaC, which are required for the synthesis of PHB and selectable marker gene, bar, for herbicide Basta resistant, were transformed into embryogenic calli. A number of transformed embryogenic lines resistant to herbicide Basta were obtained and were later regenerated to produce few hundred plantlets. Molecular analyses, including polymerase chain reaction (PCR), Southern blot, and real-time PCR have demonstrated stable integration and expression of the transgenes in the oil palm genome. HPLC and Nile blue A staining analyses confirmed the synthesis of PHB in some of the plantlets. Frontiers Media S.A. 2015-08-11 /pmc/articles/PMC4531230/ /pubmed/26322053 http://dx.doi.org/10.3389/fpls.2015.00598 Text en Copyright © 2015 Parveez, Bahariah, Ayub, Masani, Rasid, Tarmizi and Ishak. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Parveez, Ghulam Kadir Ahmad
Bahariah, Bohari
Ayub, Nor Hanin
Masani, Mat Yunus Abdul
Rasid, Omar Abdul
Tarmizi, Ahmad Hashim
Ishak, Zamzuri
Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli
title Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli
title_full Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli
title_fullStr Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli
title_full_unstemmed Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli
title_short Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli
title_sort production of polyhydroxybutyrate in oil palm (elaeis guineensis jacq.) mediated by microprojectile bombardment of phb biosynthesis genes into embryogenic calli
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531230/
https://www.ncbi.nlm.nih.gov/pubmed/26322053
http://dx.doi.org/10.3389/fpls.2015.00598
work_keys_str_mv AT parveezghulamkadirahmad productionofpolyhydroxybutyrateinoilpalmelaeisguineensisjacqmediatedbymicroprojectilebombardmentofphbbiosynthesisgenesintoembryogeniccalli
AT bahariahbohari productionofpolyhydroxybutyrateinoilpalmelaeisguineensisjacqmediatedbymicroprojectilebombardmentofphbbiosynthesisgenesintoembryogeniccalli
AT ayubnorhanin productionofpolyhydroxybutyrateinoilpalmelaeisguineensisjacqmediatedbymicroprojectilebombardmentofphbbiosynthesisgenesintoembryogeniccalli
AT masanimatyunusabdul productionofpolyhydroxybutyrateinoilpalmelaeisguineensisjacqmediatedbymicroprojectilebombardmentofphbbiosynthesisgenesintoembryogeniccalli
AT rasidomarabdul productionofpolyhydroxybutyrateinoilpalmelaeisguineensisjacqmediatedbymicroprojectilebombardmentofphbbiosynthesisgenesintoembryogeniccalli
AT tarmiziahmadhashim productionofpolyhydroxybutyrateinoilpalmelaeisguineensisjacqmediatedbymicroprojectilebombardmentofphbbiosynthesisgenesintoembryogeniccalli
AT ishakzamzuri productionofpolyhydroxybutyrateinoilpalmelaeisguineensisjacqmediatedbymicroprojectilebombardmentofphbbiosynthesisgenesintoembryogeniccalli