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Fluorogenic Assay for Inhibitors of HIV-1 Protease with Sub-picomolar Affinity
A fluorogenic substrate for HIV-1 protease was designed and used as the basis for a hypersensitive assay. The substrate exhibits a k(cat) of 7.4 s(−1), K(M) of 15 μM, and an increase in fluorescence intensity of 104-fold upon cleavage, thus providing sensitivity that is unmatched in a continuous ass...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531283/ https://www.ncbi.nlm.nih.gov/pubmed/26261098 http://dx.doi.org/10.1038/srep11286 |