Cargando…

Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome

BACKGROUND : Determination of vacA mosaicism may be important because specific Helicobacter pylori vacA genotype can be used to predict different clinical outcome. The aim of this study was to assess the relationship of vacA genotypes of Helicobacter pylori to cagA status and its development of pept...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Sill Moo, Park, Joongwon, Kim, Jae Gyu, Yoo, Byung Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association of Internal Medicine 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531701/
https://www.ncbi.nlm.nih.gov/pubmed/11417307
http://dx.doi.org/10.3904/kjim.2001.16.1.8
_version_ 1782385095545454592
author Park, Sill Moo
Park, Joongwon
Kim, Jae Gyu
Yoo, Byung Chul
author_facet Park, Sill Moo
Park, Joongwon
Kim, Jae Gyu
Yoo, Byung Chul
author_sort Park, Sill Moo
collection PubMed
description BACKGROUND : Determination of vacA mosaicism may be important because specific Helicobacter pylori vacA genotype can be used to predict different clinical outcome. The aim of this study was to assess the relationship of vacA genotypes of Helicobacter pylori to cagA status and its development of peptic ulcer diseases in Korean patients. METHODS : Gastric biopsy specimens were obtained from 53 patients with gastric ulcer (GU), 57 with duodenal ulcer (DU) and 26 with chronic gastritis (CG) patients; all patients were infected with Helicobacter pylori. Bacterial mRNAs in the gastric mucosa were amplified by RT-PCR, using synthetic oligonucleotide primers specific for the vacA and the cagA gene. Patients with vacA s1 subtype were further examined to determine whether they had s1a or s1b subtype. RESULTS : There was no correlation in frequency of vacA s1 and/or s1a genotype between CG and either GU or DU, as the vacA s1 and s1a/m1 were present in the majority of strains independent of clinical status (s1 ; 100.0% versus 94.3 % or 93.0 % and s1a/m1 : 76.9% versus 62.3% or 64.9%, respectively). Likewise, there was no difference in the prevalence of the cagA gene between CG and either GU or DU patients (92.3% versus 90.6% or 98.2%, respectively). In addition, the cagA-negative status did not predict the presence of vacA s2 genotype. CONCLUSION : These results strongly suggest that either cagA or vacA s1 and/or s1a is not proved to be a useful marker to distinguish disease-specific Helicobacter pylori strains for the development of peptic ulcer diseases in Korean patients.
format Online
Article
Text
id pubmed-4531701
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Korean Association of Internal Medicine
record_format MEDLINE/PubMed
spelling pubmed-45317012015-10-02 Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome Park, Sill Moo Park, Joongwon Kim, Jae Gyu Yoo, Byung Chul Korean J Intern Med Original Article BACKGROUND : Determination of vacA mosaicism may be important because specific Helicobacter pylori vacA genotype can be used to predict different clinical outcome. The aim of this study was to assess the relationship of vacA genotypes of Helicobacter pylori to cagA status and its development of peptic ulcer diseases in Korean patients. METHODS : Gastric biopsy specimens were obtained from 53 patients with gastric ulcer (GU), 57 with duodenal ulcer (DU) and 26 with chronic gastritis (CG) patients; all patients were infected with Helicobacter pylori. Bacterial mRNAs in the gastric mucosa were amplified by RT-PCR, using synthetic oligonucleotide primers specific for the vacA and the cagA gene. Patients with vacA s1 subtype were further examined to determine whether they had s1a or s1b subtype. RESULTS : There was no correlation in frequency of vacA s1 and/or s1a genotype between CG and either GU or DU, as the vacA s1 and s1a/m1 were present in the majority of strains independent of clinical status (s1 ; 100.0% versus 94.3 % or 93.0 % and s1a/m1 : 76.9% versus 62.3% or 64.9%, respectively). Likewise, there was no difference in the prevalence of the cagA gene between CG and either GU or DU patients (92.3% versus 90.6% or 98.2%, respectively). In addition, the cagA-negative status did not predict the presence of vacA s2 genotype. CONCLUSION : These results strongly suggest that either cagA or vacA s1 and/or s1a is not proved to be a useful marker to distinguish disease-specific Helicobacter pylori strains for the development of peptic ulcer diseases in Korean patients. Korean Association of Internal Medicine 2001-03 /pmc/articles/PMC4531701/ /pubmed/11417307 http://dx.doi.org/10.3904/kjim.2001.16.1.8 Text en Copyright © 2001 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Sill Moo
Park, Joongwon
Kim, Jae Gyu
Yoo, Byung Chul
Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome
title Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome
title_full Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome
title_fullStr Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome
title_full_unstemmed Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome
title_short Relevance of vacA Genotypes of Helicobacter pylori to cagA Status and Its Clinical Outcome
title_sort relevance of vaca genotypes of helicobacter pylori to caga status and its clinical outcome
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531701/
https://www.ncbi.nlm.nih.gov/pubmed/11417307
http://dx.doi.org/10.3904/kjim.2001.16.1.8
work_keys_str_mv AT parksillmoo relevanceofvacagenotypesofhelicobacterpyloritocagastatusanditsclinicaloutcome
AT parkjoongwon relevanceofvacagenotypesofhelicobacterpyloritocagastatusanditsclinicaloutcome
AT kimjaegyu relevanceofvacagenotypesofhelicobacterpyloritocagastatusanditsclinicaloutcome
AT yoobyungchul relevanceofvacagenotypesofhelicobacterpyloritocagastatusanditsclinicaloutcome