The impact of read length on quantification of differentially expressed genes and splice junction detection

BACKGROUND: The initial next-generation sequencing technologies produced reads of 25 or 36 bp, and only from a single-end of the library sequence. Currently, it is possible to reliably produce 300 bp paired-end sequences for RNA expression analysis. While read lengths have consistently increased, pe...

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Detalles Bibliográficos
Autores principales: Chhangawala, Sagar, Rudy, Gabe, Mason, Christopher E., Rosenfeld, Jeffrey A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531809/
https://www.ncbi.nlm.nih.gov/pubmed/26100517
http://dx.doi.org/10.1186/s13059-015-0697-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531809/
https://www.ncbi.nlm.nih.gov/pubmed/26100517
http://dx.doi.org/10.1186/s13059-015-0697-y

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