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The impact of read length on quantification of differentially expressed genes and splice junction detection
BACKGROUND: The initial next-generation sequencing technologies produced reads of 25 or 36 bp, and only from a single-end of the library sequence. Currently, it is possible to reliably produce 300 bp paired-end sequences for RNA expression analysis. While read lengths have consistently increased, pe...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531809/ https://www.ncbi.nlm.nih.gov/pubmed/26100517 http://dx.doi.org/10.1186/s13059-015-0697-y |