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Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells
OBJECTIVES: In HFRS, there is a varying degree of disseminated intravascular coagulation which was evident in the early phase of the illness. It is believed also that DIC would be the consequence, at least in part, of functional changes of endothelium resulting in kinin activation and clinical syndr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association of Internal Medicine
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531915/ https://www.ncbi.nlm.nih.gov/pubmed/10461425 http://dx.doi.org/10.3904/kjim.1999.14.2.47 |
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author | Song, Jeong-Soo Min, Cheol-Hong Kang, Eungtaek Yu, Suk-Hee |
author_facet | Song, Jeong-Soo Min, Cheol-Hong Kang, Eungtaek Yu, Suk-Hee |
author_sort | Song, Jeong-Soo |
collection | PubMed |
description | OBJECTIVES: In HFRS, there is a varying degree of disseminated intravascular coagulation which was evident in the early phase of the illness. It is believed also that DIC would be the consequence, at least in part, of functional changes of endothelium resulting in kinin activation and clinical syndrome. This study investigated the role of adhesion molecule in the pathogenesis of Hantaan virus-related disease. METHODS: The expression of ICAM-1 antigen on the cell membrane of human umbilical vein endothelial cells was assessed by immunohistochemistry, and ICAM-1 mRNA in the endothelial cells was assessed by in situ hybridization after Hantaan virus infection (2.6 × 10(4) PFU/mL) with the time course. RESULTS: In immunohistochemistry, the number of ICAM-1 positive cells increased with time during the 12 or 24 hours after infection. 5 to 10 % of HUVECs had been positive after 12 – 24 hours and the number of positive cells decreased abruptly after 24 hours. Hantaan antigen had been noticed after 12 hours focally on the HUVECs but continued to proliferate into day 7 post-infection when most of HUVECs were infected by Hantaan virus, in situ hybridization showed identical patterns of ICAM-1 mRNA expression after Hantaan virus infection. CONCLUSION: It implies that the Hantaan virus infection on HUVECs would express more ICAM-1 on their surface and implicated in the pathogenesis of early clinical syndrome of HFRS. |
format | Online Article Text |
id | pubmed-4531915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-45319152015-10-02 Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells Song, Jeong-Soo Min, Cheol-Hong Kang, Eungtaek Yu, Suk-Hee Korean J Intern Med Original Article OBJECTIVES: In HFRS, there is a varying degree of disseminated intravascular coagulation which was evident in the early phase of the illness. It is believed also that DIC would be the consequence, at least in part, of functional changes of endothelium resulting in kinin activation and clinical syndrome. This study investigated the role of adhesion molecule in the pathogenesis of Hantaan virus-related disease. METHODS: The expression of ICAM-1 antigen on the cell membrane of human umbilical vein endothelial cells was assessed by immunohistochemistry, and ICAM-1 mRNA in the endothelial cells was assessed by in situ hybridization after Hantaan virus infection (2.6 × 10(4) PFU/mL) with the time course. RESULTS: In immunohistochemistry, the number of ICAM-1 positive cells increased with time during the 12 or 24 hours after infection. 5 to 10 % of HUVECs had been positive after 12 – 24 hours and the number of positive cells decreased abruptly after 24 hours. Hantaan antigen had been noticed after 12 hours focally on the HUVECs but continued to proliferate into day 7 post-infection when most of HUVECs were infected by Hantaan virus, in situ hybridization showed identical patterns of ICAM-1 mRNA expression after Hantaan virus infection. CONCLUSION: It implies that the Hantaan virus infection on HUVECs would express more ICAM-1 on their surface and implicated in the pathogenesis of early clinical syndrome of HFRS. Korean Association of Internal Medicine 1999-07 /pmc/articles/PMC4531915/ /pubmed/10461425 http://dx.doi.org/10.3904/kjim.1999.14.2.47 Text en Copyright © 1999 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Song, Jeong-Soo Min, Cheol-Hong Kang, Eungtaek Yu, Suk-Hee Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells |
title | Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells |
title_full | Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells |
title_fullStr | Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells |
title_full_unstemmed | Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells |
title_short | Expression of ICAM-1 on the Hantaan Virus-Infected Human Umbilical Vein Endothelial Cells |
title_sort | expression of icam-1 on the hantaan virus-infected human umbilical vein endothelial cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531915/ https://www.ncbi.nlm.nih.gov/pubmed/10461425 http://dx.doi.org/10.3904/kjim.1999.14.2.47 |
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