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Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease

OBJECTIVES: Immunologic studies have characterized the numbers and types of inflammatory cells in diseased inflammatory bowel disease (IBD) mucosa but have yielded conflicting results regarding intestinal lymphocytes activation in IBD. We investigated the levels of lymphocytes subsets, interieukin-2...

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Autores principales: Lee, Heung Bum, Kim, Jong Hun, Yim, Chang Yeol, Kim, Dae Ghon, Ahn, Deuk Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association of Internal Medicine 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531961/
https://www.ncbi.nlm.nih.gov/pubmed/9159031
http://dx.doi.org/10.3904/kjim.1997.12.1.7
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author Lee, Heung Bum
Kim, Jong Hun
Yim, Chang Yeol
Kim, Dae Ghon
Ahn, Deuk Soo
author_facet Lee, Heung Bum
Kim, Jong Hun
Yim, Chang Yeol
Kim, Dae Ghon
Ahn, Deuk Soo
author_sort Lee, Heung Bum
collection PubMed
description OBJECTIVES: Immunologic studies have characterized the numbers and types of inflammatory cells in diseased inflammatory bowel disease (IBD) mucosa but have yielded conflicting results regarding intestinal lymphocytes activation in IBD. We investigated the levels of lymphocytes subsets, interieukin-2 receptor, transferrin receptor, and T cell receptors in mainly isolated lamina propria lymphocytes, including intraepithelial lymphocytes of normal colonic mucosa or IBD (ulcerative colitis and Crohn’s disease) mucosa to understand the pathogenesis of IBD. We have results from this study. RESULTS: 1) In comparing ulcerative colitis with control, IL-2R (p<0.05), TR (p<0.01), and CD3/HLA-DR (p<0.05) showed a significant increase. 2) In comparing Crohn’s disease with control, CD3 (p<0.05), TCR α/β (p<0.01) and TCR γ/δ (p<0.05) showed a significant decrease. 3) In comparing Crohn’s disease with ulcerative colitis, CD19 (p<0.01), TR (p< 0.01), TCR α/β (p<0.01) and TCR γ/δ (p<0.05) showed a significant decrease. CONCLUSION: From these results, there are increased T cell markers, IL-2R, TR, and CD3/HLA-DR in UC, but differently, decreased CD3, TCR α/β and TCR γ/δ in CD compared with control. In addition, definitive differences in lymphocytes markers, CD19, TR, TCR α/β and TCR γ/δ, which are higher in UC than in CD, may elucidate the different immunopathogenesis between UC and CD.
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spelling pubmed-45319612015-10-02 Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease Lee, Heung Bum Kim, Jong Hun Yim, Chang Yeol Kim, Dae Ghon Ahn, Deuk Soo Korean J Intern Med Original Article OBJECTIVES: Immunologic studies have characterized the numbers and types of inflammatory cells in diseased inflammatory bowel disease (IBD) mucosa but have yielded conflicting results regarding intestinal lymphocytes activation in IBD. We investigated the levels of lymphocytes subsets, interieukin-2 receptor, transferrin receptor, and T cell receptors in mainly isolated lamina propria lymphocytes, including intraepithelial lymphocytes of normal colonic mucosa or IBD (ulcerative colitis and Crohn’s disease) mucosa to understand the pathogenesis of IBD. We have results from this study. RESULTS: 1) In comparing ulcerative colitis with control, IL-2R (p<0.05), TR (p<0.01), and CD3/HLA-DR (p<0.05) showed a significant increase. 2) In comparing Crohn’s disease with control, CD3 (p<0.05), TCR α/β (p<0.01) and TCR γ/δ (p<0.05) showed a significant decrease. 3) In comparing Crohn’s disease with ulcerative colitis, CD19 (p<0.01), TR (p< 0.01), TCR α/β (p<0.01) and TCR γ/δ (p<0.05) showed a significant decrease. CONCLUSION: From these results, there are increased T cell markers, IL-2R, TR, and CD3/HLA-DR in UC, but differently, decreased CD3, TCR α/β and TCR γ/δ in CD compared with control. In addition, definitive differences in lymphocytes markers, CD19, TR, TCR α/β and TCR γ/δ, which are higher in UC than in CD, may elucidate the different immunopathogenesis between UC and CD. Korean Association of Internal Medicine 1997-01 /pmc/articles/PMC4531961/ /pubmed/9159031 http://dx.doi.org/10.3904/kjim.1997.12.1.7 Text en Copyright © 1997 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Heung Bum
Kim, Jong Hun
Yim, Chang Yeol
Kim, Dae Ghon
Ahn, Deuk Soo
Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease
title Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease
title_full Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease
title_fullStr Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease
title_full_unstemmed Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease
title_short Differences in Immunophenotyping of Mucosal Lymphocytes Between Ulcerative Colitis and Crohn’s Disease
title_sort differences in immunophenotyping of mucosal lymphocytes between ulcerative colitis and crohn’s disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531961/
https://www.ncbi.nlm.nih.gov/pubmed/9159031
http://dx.doi.org/10.3904/kjim.1997.12.1.7
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