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Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations
OBJECTIVES: Since conventional cytogenetic analysis for bronchogenic carcinogenesis is limited by the difficulty to get enough number of high quality metaphase spreads, the development of new method to overcome above problems is strongly needed. Therefore, the introduction of non-radioactive in situ...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association of Internal Medicine
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532067/ https://www.ncbi.nlm.nih.gov/pubmed/7865489 http://dx.doi.org/10.3904/kjim.1994.9.2.55 |
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author | Kim, Sun Young Lee, Kyoung Joo Hong, Seok Cheol Han, Pyo Seong Lee, Jong Jin Cho, Hai Jeong Kim, Ae Kyoung Kim, Ju Ock Lee, Mi Seon |
author_facet | Kim, Sun Young Lee, Kyoung Joo Hong, Seok Cheol Han, Pyo Seong Lee, Jong Jin Cho, Hai Jeong Kim, Ae Kyoung Kim, Ju Ock Lee, Mi Seon |
author_sort | Kim, Sun Young |
collection | PubMed |
description | OBJECTIVES: Since conventional cytogenetic analysis for bronchogenic carcinogenesis is limited by the difficulty to get enough number of high quality metaphase spreads, the development of new method to overcome above problems is strongly needed. Therefore, the introduction of non-radioactive in situ hybridization (ISH) with pericentromeric chromosome probes gave us the way to investigate the genetic events during carcinogenic process. We applied this method on lung cancer tissue to validate the possibility of this method for general usage and to analyze numerical chromosome aberration status and their clinical correlations. METHODS: A set of satellite DNA probes specific for chromosome 3, 7, 9, 11, and 17 was hybridized directly to paraffin-embedded tissue section of 30 non-small cell lung cancers. Mean chromosome index of each chromosome and frequency of polysomy for each chromosome were calculated. RESULTS: Mean chromosome indices for chromosome 3, 7, 9, 11, and 17 were 1.10, 1.13, 1.17, 1.12, and 1.17. respectively. Polysomy for a set of chromosomes was detected in all 30 cases except 4 cases which showed hypoploidy only for chromosome 3 or 7 in 2 cases and diploidy only for chromosome 3 or 11 in 2 cases. Among the set of chromosomes, mean chromosome index and polysomy frequency for chromosome 9 & 17 were significantly higher than that for others. Mean chromosome index or polysomy pattern for each chromosome was not much different among cell types or clinical stages. CONCLUSIONS: Our results show that chromosome ISH can be used to screen for numerical chromosome aberrations on paraffin tissue sections and further studies for ISH analysis with different probes on same tumor area or double-target ISH in large scale are needed to confirm above results and to elucidate the specific meanings. |
format | Online Article Text |
id | pubmed-4532067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-45320672015-10-02 Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations Kim, Sun Young Lee, Kyoung Joo Hong, Seok Cheol Han, Pyo Seong Lee, Jong Jin Cho, Hai Jeong Kim, Ae Kyoung Kim, Ju Ock Lee, Mi Seon Korean J Intern Med Original Article OBJECTIVES: Since conventional cytogenetic analysis for bronchogenic carcinogenesis is limited by the difficulty to get enough number of high quality metaphase spreads, the development of new method to overcome above problems is strongly needed. Therefore, the introduction of non-radioactive in situ hybridization (ISH) with pericentromeric chromosome probes gave us the way to investigate the genetic events during carcinogenic process. We applied this method on lung cancer tissue to validate the possibility of this method for general usage and to analyze numerical chromosome aberration status and their clinical correlations. METHODS: A set of satellite DNA probes specific for chromosome 3, 7, 9, 11, and 17 was hybridized directly to paraffin-embedded tissue section of 30 non-small cell lung cancers. Mean chromosome index of each chromosome and frequency of polysomy for each chromosome were calculated. RESULTS: Mean chromosome indices for chromosome 3, 7, 9, 11, and 17 were 1.10, 1.13, 1.17, 1.12, and 1.17. respectively. Polysomy for a set of chromosomes was detected in all 30 cases except 4 cases which showed hypoploidy only for chromosome 3 or 7 in 2 cases and diploidy only for chromosome 3 or 11 in 2 cases. Among the set of chromosomes, mean chromosome index and polysomy frequency for chromosome 9 & 17 were significantly higher than that for others. Mean chromosome index or polysomy pattern for each chromosome was not much different among cell types or clinical stages. CONCLUSIONS: Our results show that chromosome ISH can be used to screen for numerical chromosome aberrations on paraffin tissue sections and further studies for ISH analysis with different probes on same tumor area or double-target ISH in large scale are needed to confirm above results and to elucidate the specific meanings. Korean Association of Internal Medicine 1994-07 /pmc/articles/PMC4532067/ /pubmed/7865489 http://dx.doi.org/10.3904/kjim.1994.9.2.55 Text en Copyright © 1994 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Sun Young Lee, Kyoung Joo Hong, Seok Cheol Han, Pyo Seong Lee, Jong Jin Cho, Hai Jeong Kim, Ae Kyoung Kim, Ju Ock Lee, Mi Seon Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations |
title | Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations |
title_full | Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations |
title_fullStr | Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations |
title_full_unstemmed | Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations |
title_short | Interphase Cytogenetics of Lung Tumors Using In Situ Hybridization: Numerical Aberrations |
title_sort | interphase cytogenetics of lung tumors using in situ hybridization: numerical aberrations |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532067/ https://www.ncbi.nlm.nih.gov/pubmed/7865489 http://dx.doi.org/10.3904/kjim.1994.9.2.55 |
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