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Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease

The authors investigated the distribution of HCV genotypes in patients with various chronic liver diseases in Korea. Study population was 70 individulals, positive for second generation anti-HCV EIA, consisting of 37 cases with sporadic non-A, non-B (NANB) chronic hepatitis (CH), 12 NANB hepatocellu...

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Autores principales: Yoon, Seung Kew, Park, Young Min, Chung, Kyu Won, Kim, Boo Sung, Kim, Chul Joong, Kim, Won Young, Chang, Seung Kee, Cho, Myung Jae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association of Internal Medicine 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532084/
https://www.ncbi.nlm.nih.gov/pubmed/7518242
http://dx.doi.org/10.3904/kjim.1993.8.2.66
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author Yoon, Seung Kew
Park, Young Min
Chung, Kyu Won
Kim, Boo Sung
Kim, Chul Joong
Kim, Won Young
Chang, Seung Kee
Cho, Myung Jae
author_facet Yoon, Seung Kew
Park, Young Min
Chung, Kyu Won
Kim, Boo Sung
Kim, Chul Joong
Kim, Won Young
Chang, Seung Kee
Cho, Myung Jae
author_sort Yoon, Seung Kew
collection PubMed
description The authors investigated the distribution of HCV genotypes in patients with various chronic liver diseases in Korea. Study population was 70 individulals, positive for second generation anti-HCV EIA, consisting of 37 cases with sporadic non-A, non-B (NANB) chronic hepatitis (CH), 12 NANB hepatocellular carcinoma, 16 post-transfusion NANB hepatitis, 4 non-B blood donors and 1 healthy family member of a patient with sporadic CH. Molecular typing was performed by RT-nested PCR with type-specific primer sets deduced from the NS-5 region of HCV. The prevalence of type II was 75.0% and type III was 25.0% in sera. In liver tissues, type II HCV was shown in 63.0%, type III HCV in 3.7% and co-infections with type II and III HCV were observed in 18.5% of 27 samples biopsied. In the sera of patients with chronic hepatitis, typing results were relatively well correlated with those in tissues (75%), but type III could not be observed. Among 12 HCC patients, type III HCV appeared only in tissues, not in sera. These results suggest that type II HCV may be the major HCV type in Korea, and co-infections with type II and-III HCV may not be rare in chronic liver diseases with HCV.
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spelling pubmed-45320842015-10-02 Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease Yoon, Seung Kew Park, Young Min Chung, Kyu Won Kim, Boo Sung Kim, Chul Joong Kim, Won Young Chang, Seung Kee Cho, Myung Jae Korean J Intern Med Original Article The authors investigated the distribution of HCV genotypes in patients with various chronic liver diseases in Korea. Study population was 70 individulals, positive for second generation anti-HCV EIA, consisting of 37 cases with sporadic non-A, non-B (NANB) chronic hepatitis (CH), 12 NANB hepatocellular carcinoma, 16 post-transfusion NANB hepatitis, 4 non-B blood donors and 1 healthy family member of a patient with sporadic CH. Molecular typing was performed by RT-nested PCR with type-specific primer sets deduced from the NS-5 region of HCV. The prevalence of type II was 75.0% and type III was 25.0% in sera. In liver tissues, type II HCV was shown in 63.0%, type III HCV in 3.7% and co-infections with type II and III HCV were observed in 18.5% of 27 samples biopsied. In the sera of patients with chronic hepatitis, typing results were relatively well correlated with those in tissues (75%), but type III could not be observed. Among 12 HCC patients, type III HCV appeared only in tissues, not in sera. These results suggest that type II HCV may be the major HCV type in Korea, and co-infections with type II and-III HCV may not be rare in chronic liver diseases with HCV. Korean Association of Internal Medicine 1993-07 /pmc/articles/PMC4532084/ /pubmed/7518242 http://dx.doi.org/10.3904/kjim.1993.8.2.66 Text en Copyright © 1993 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yoon, Seung Kew
Park, Young Min
Chung, Kyu Won
Kim, Boo Sung
Kim, Chul Joong
Kim, Won Young
Chang, Seung Kee
Cho, Myung Jae
Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease
title Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease
title_full Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease
title_fullStr Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease
title_full_unstemmed Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease
title_short Molecular Typing of Hepatitis C Virus Genome from Sera and Liver Tissues of Patients with anti-HCV Positive Chronic Liver Disease
title_sort molecular typing of hepatitis c virus genome from sera and liver tissues of patients with anti-hcv positive chronic liver disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532084/
https://www.ncbi.nlm.nih.gov/pubmed/7518242
http://dx.doi.org/10.3904/kjim.1993.8.2.66
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