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The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction
In order to evaluate the influence of dilution, pH and ionic strength on the precipitation of t-PA and PAI-1 during euglobulin precipitation, we measured t-PA Ag, PAI-1 Ag and fibrinolytic activity in the euglobulin fraction made of pooled plasma from liver cirrhosis patients, under various conditio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association of Internal Medicine
1992
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532111/ https://www.ncbi.nlm.nih.gov/pubmed/1306075 http://dx.doi.org/10.3904/kjim.1992.7.2.73 |
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author | Hong, Sae Yong Shin, Hyun Kill Yang, Dong Ho |
author_facet | Hong, Sae Yong Shin, Hyun Kill Yang, Dong Ho |
author_sort | Hong, Sae Yong |
collection | PubMed |
description | In order to evaluate the influence of dilution, pH and ionic strength on the precipitation of t-PA and PAI-1 during euglobulin precipitation, we measured t-PA Ag, PAI-1 Ag and fibrinolytic activity in the euglobulin fraction made of pooled plasma from liver cirrhosis patients, under various conditions by changing pH, ionic strength and degree of dilution. The precipitation of t-PA Ag in the euglobulin fraction was enhanced by decreasing the ionic strength and greatest at pH 6.0. The fibrinolytic activity in the euglobulin fraction showed consistent changes with t-PA Ag under varying pH and ionic strength. The precipitation of t-PA Ag was not influenced by the dilution factor but the larger the dilution factor, the greater the PAI-1 and the smaller the fibrinolytic activity in the euglobulin fraction. PAI Ag in euglobulin fraction showed consistent changes with t-PA Ag in the euglobulin fraction regardless of the changes in ionic strength and pH. The amount of precipitation of t-PA and PAI-1 was increased by the presence of dextran sulfate, under varying pH, ionic strength and dilution conditions. Our results show theat the currently used conditions for standard euglobulin precipitation are the most favorable for t-PA precipitation into the euglobulin fraction. The fibrinolytic activity exerted in the euglobulin fraction seems to depend on the amout of t-PA-PAI-1 complex rather than minimized protease inhibitor in the euglobulin fraction. |
format | Online Article Text |
id | pubmed-4532111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-45321112015-10-02 The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction Hong, Sae Yong Shin, Hyun Kill Yang, Dong Ho Korean J Intern Med Articles In order to evaluate the influence of dilution, pH and ionic strength on the precipitation of t-PA and PAI-1 during euglobulin precipitation, we measured t-PA Ag, PAI-1 Ag and fibrinolytic activity in the euglobulin fraction made of pooled plasma from liver cirrhosis patients, under various conditions by changing pH, ionic strength and degree of dilution. The precipitation of t-PA Ag in the euglobulin fraction was enhanced by decreasing the ionic strength and greatest at pH 6.0. The fibrinolytic activity in the euglobulin fraction showed consistent changes with t-PA Ag under varying pH and ionic strength. The precipitation of t-PA Ag was not influenced by the dilution factor but the larger the dilution factor, the greater the PAI-1 and the smaller the fibrinolytic activity in the euglobulin fraction. PAI Ag in euglobulin fraction showed consistent changes with t-PA Ag in the euglobulin fraction regardless of the changes in ionic strength and pH. The amount of precipitation of t-PA and PAI-1 was increased by the presence of dextran sulfate, under varying pH, ionic strength and dilution conditions. Our results show theat the currently used conditions for standard euglobulin precipitation are the most favorable for t-PA precipitation into the euglobulin fraction. The fibrinolytic activity exerted in the euglobulin fraction seems to depend on the amout of t-PA-PAI-1 complex rather than minimized protease inhibitor in the euglobulin fraction. Korean Association of Internal Medicine 1992-07 /pmc/articles/PMC4532111/ /pubmed/1306075 http://dx.doi.org/10.3904/kjim.1992.7.2.73 Text en Copyright © 1992 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Hong, Sae Yong Shin, Hyun Kill Yang, Dong Ho The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction |
title | The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction |
title_full | The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction |
title_fullStr | The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction |
title_full_unstemmed | The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction |
title_short | The Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction |
title_sort | effect of dilution, ph and ionic strength of plasma on t-pa precipitation in euglobulin fraction |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532111/ https://www.ncbi.nlm.nih.gov/pubmed/1306075 http://dx.doi.org/10.3904/kjim.1992.7.2.73 |
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