Large-volume en-bloc staining for electron microscopy-based connectomics

Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM...

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Detalles Bibliográficos
Autores principales: Hua, Yunfeng, Laserstein, Philip, Helmstaedter, Moritz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532871/
https://www.ncbi.nlm.nih.gov/pubmed/26235643
http://dx.doi.org/10.1038/ncomms8923

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532871/
https://www.ncbi.nlm.nih.gov/pubmed/26235643
http://dx.doi.org/10.1038/ncomms8923

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