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Quadruplex-and-Mg(2+) Connection (QMC) of DNA

This work highlights a novel method of coupling of nucleic acids through formation of an extraordinary stable, specific and fully reversible quadruplex-and-Mg(2+) connection (QMC). QMC employs the monomolecular tetrahelical architecture of DNA and has two key components: (i) shape complementarity be...

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Autor principal: Kankia, Besik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4533012/
https://www.ncbi.nlm.nih.gov/pubmed/26265243
http://dx.doi.org/10.1038/srep12996
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author Kankia, Besik
author_facet Kankia, Besik
author_sort Kankia, Besik
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description This work highlights a novel method of coupling of nucleic acids through formation of an extraordinary stable, specific and fully reversible quadruplex-and-Mg(2+) connection (QMC). QMC employs the monomolecular tetrahelical architecture of DNA and has two key components: (i) shape complementarity between QMC partners, which is introduced by specific modifications of the quadruplexes, and (ii) Mg(2+) ions. The on-rate of QMC formation is between 10(5)–10(6) M(−1) s(−1), while the off-rate is undetectable even at 80 °C. However, QMC dissociates rapidly upon removal of Mg(2+) ions (i.e., by EDTA). QMC is characterized by high specificity, as even a single-nucleotide modification of one of the connectors inhibits complex-formation. QMC has the potential to revolutionize biotechnology by introducing a new class of capture molecules with major advantages over traditional systems such as streptavidin-biotin. The advantages include reversibility, multiplexing, higher stability and specificity, longer shelf life and low cost.
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spelling pubmed-45330122015-08-13 Quadruplex-and-Mg(2+) Connection (QMC) of DNA Kankia, Besik Sci Rep Article This work highlights a novel method of coupling of nucleic acids through formation of an extraordinary stable, specific and fully reversible quadruplex-and-Mg(2+) connection (QMC). QMC employs the monomolecular tetrahelical architecture of DNA and has two key components: (i) shape complementarity between QMC partners, which is introduced by specific modifications of the quadruplexes, and (ii) Mg(2+) ions. The on-rate of QMC formation is between 10(5)–10(6) M(−1) s(−1), while the off-rate is undetectable even at 80 °C. However, QMC dissociates rapidly upon removal of Mg(2+) ions (i.e., by EDTA). QMC is characterized by high specificity, as even a single-nucleotide modification of one of the connectors inhibits complex-formation. QMC has the potential to revolutionize biotechnology by introducing a new class of capture molecules with major advantages over traditional systems such as streptavidin-biotin. The advantages include reversibility, multiplexing, higher stability and specificity, longer shelf life and low cost. Nature Publishing Group 2015-08-12 /pmc/articles/PMC4533012/ /pubmed/26265243 http://dx.doi.org/10.1038/srep12996 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kankia, Besik
Quadruplex-and-Mg(2+) Connection (QMC) of DNA
title Quadruplex-and-Mg(2+) Connection (QMC) of DNA
title_full Quadruplex-and-Mg(2+) Connection (QMC) of DNA
title_fullStr Quadruplex-and-Mg(2+) Connection (QMC) of DNA
title_full_unstemmed Quadruplex-and-Mg(2+) Connection (QMC) of DNA
title_short Quadruplex-and-Mg(2+) Connection (QMC) of DNA
title_sort quadruplex-and-mg(2+) connection (qmc) of dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4533012/
https://www.ncbi.nlm.nih.gov/pubmed/26265243
http://dx.doi.org/10.1038/srep12996
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