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Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210 °C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using Cellic® CTec 2 (20 U or 0.87 FPU g(−1) substrate) cellulase plus β-glucosi...

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Detalles Bibliográficos
Autores principales: Zhao, X., Moates, G.K., Elliston, A., Wilson, D.R., Coleman, M.J., Waldron, K.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Applied Science ;, Elsevier Science Pub. Co 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534771/
https://www.ncbi.nlm.nih.gov/pubmed/26210138
http://dx.doi.org/10.1016/j.biortech.2015.06.131
Descripción
Sumario:This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210 °C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using Cellic® CTec 2 (20 U or 0.87 FPU g(−1) substrate) cellulase plus β-glucosidase (2 U g(−1) substrate) and a yeast inoculum of 10% (v/v or 8.0 × 10(7) cells mL(−1)). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concentration of 20% (w/v) by metabolising fermentation inhibitors.