The immune adherence receptor CR1-like existed on porcine erythrocytes membrane

In the present study, we obtain a mouse anti-porcine complement receptor type 1 (CR1)-like monoclonal antibody (McAb) and use this McAb to verify the existence of CR1-like protein on porcine erythrocytes. Our results confirm that CR1-like protein is localized on the surface of porcine erythrocytes....

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Autores principales: Yin, Wei, Cui, Jiaoyan, Jiang, Junbing, Zhao, Junxing, Fan, Kuohai, Sun, Na, Wang, Zhiwei, Sun, Yaogui, Ma, Haili, Li, Hongquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534784/
https://www.ncbi.nlm.nih.gov/pubmed/26268676
http://dx.doi.org/10.1038/srep13290
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author Yin, Wei
Cui, Jiaoyan
Jiang, Junbing
Zhao, Junxing
Fan, Kuohai
Sun, Na
Wang, Zhiwei
Sun, Yaogui
Ma, Haili
Li, Hongquan
author_facet Yin, Wei
Cui, Jiaoyan
Jiang, Junbing
Zhao, Junxing
Fan, Kuohai
Sun, Na
Wang, Zhiwei
Sun, Yaogui
Ma, Haili
Li, Hongquan
author_sort Yin, Wei
collection PubMed
description In the present study, we obtain a mouse anti-porcine complement receptor type 1 (CR1)-like monoclonal antibody (McAb) and use this McAb to verify the existence of CR1-like protein on porcine erythrocytes. Our results confirm that CR1-like protein is localized on the surface of porcine erythrocytes. Mouse immunoglobulin G inhibited the binding of serum-opsonized green fluorescent protein-expressing Escherichia coli to porcine erythrocytes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicates that CR1-like McAb reacts with biochemically-purified porcine erythrocyte membrane fractions, with a clear band at 135 kDa to 140 kDa. We postulate that the 135 kDa to 140 kDa membrane protein is the equivalent of the porcine erythrocyte CR1-like protein.
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spelling pubmed-45347842015-08-21 The immune adherence receptor CR1-like existed on porcine erythrocytes membrane Yin, Wei Cui, Jiaoyan Jiang, Junbing Zhao, Junxing Fan, Kuohai Sun, Na Wang, Zhiwei Sun, Yaogui Ma, Haili Li, Hongquan Sci Rep Article In the present study, we obtain a mouse anti-porcine complement receptor type 1 (CR1)-like monoclonal antibody (McAb) and use this McAb to verify the existence of CR1-like protein on porcine erythrocytes. Our results confirm that CR1-like protein is localized on the surface of porcine erythrocytes. Mouse immunoglobulin G inhibited the binding of serum-opsonized green fluorescent protein-expressing Escherichia coli to porcine erythrocytes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicates that CR1-like McAb reacts with biochemically-purified porcine erythrocyte membrane fractions, with a clear band at 135 kDa to 140 kDa. We postulate that the 135 kDa to 140 kDa membrane protein is the equivalent of the porcine erythrocyte CR1-like protein. Nature Publishing Group 2015-08-13 /pmc/articles/PMC4534784/ /pubmed/26268676 http://dx.doi.org/10.1038/srep13290 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yin, Wei
Cui, Jiaoyan
Jiang, Junbing
Zhao, Junxing
Fan, Kuohai
Sun, Na
Wang, Zhiwei
Sun, Yaogui
Ma, Haili
Li, Hongquan
The immune adherence receptor CR1-like existed on porcine erythrocytes membrane
title The immune adherence receptor CR1-like existed on porcine erythrocytes membrane
title_full The immune adherence receptor CR1-like existed on porcine erythrocytes membrane
title_fullStr The immune adherence receptor CR1-like existed on porcine erythrocytes membrane
title_full_unstemmed The immune adherence receptor CR1-like existed on porcine erythrocytes membrane
title_short The immune adherence receptor CR1-like existed on porcine erythrocytes membrane
title_sort immune adherence receptor cr1-like existed on porcine erythrocytes membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534784/
https://www.ncbi.nlm.nih.gov/pubmed/26268676
http://dx.doi.org/10.1038/srep13290
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