A Simple Quantitative Assay of Vascular Plasminogen Activator: — Not Only Stimulated Firinolytic Activity But Also Basal Firinolytic Activity are Correlated with Venous Fibrinolytic Activity —

A simple method for the quantitative assay of vascular plasminogen activator is described. From 24 chronic renal failure patients who were undergoing surgery for A/V fistular or shunt construction, samll pieces of artery (mean 7.8 mg, range 3.0 – 18.5 mg) and vein (mean 4.5 mg, range 3.0 – 8.8 mg) were used. The fibrinolytic acitivity was assayed on a well-controlled fibrin plate by only distilled water immersion and incubation at 37°C. Basal fibrinolytic activity was correlated with venous fibrinolytic activity (r = 0.773, p<0.0001) and with arterial fibrinolytic activity (r = 0.55, 0.005<p<0.01). The increment of fibrinolytic activity by venous occlusion was correlated to venous fibrinolytic activity (r = 0.849, p < 0. 0001) but not to arterial fibrinolytic activity (r = 0.34, 0.1<p<0.25).