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Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics

OBJECTIVES: We set out to investigate the impact of common antibiotics on Panton–Valentine Leucocidin (PVL) expression by methicillin-sensitive Staphylococcus aureus (MSSA). PVL expression by methicillin-resistant S. aureus (MRSA) is reportedly enhanced by β-lactams, but inhibited by protein-synthes...

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Autores principales: Turner, Claire E., Sriskandan, Shiranee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: W.B. Saunders 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535317/
https://www.ncbi.nlm.nih.gov/pubmed/26028260
http://dx.doi.org/10.1016/j.jinf.2015.05.008
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author Turner, Claire E.
Sriskandan, Shiranee
author_facet Turner, Claire E.
Sriskandan, Shiranee
author_sort Turner, Claire E.
collection PubMed
description OBJECTIVES: We set out to investigate the impact of common antibiotics on Panton–Valentine Leucocidin (PVL) expression by methicillin-sensitive Staphylococcus aureus (MSSA). PVL expression by methicillin-resistant S. aureus (MRSA) is reportedly enhanced by β-lactams, but inhibited by protein-synthesis inhibitors, a fact that has influenced management of infections associated with PVL. Although PVL is more frequently associated with MSSA than MRSA in the UK, the effect of antibiotics on PVL expression by MSSA has not been fully addressed. METHODS: MSSA was cultured in vitro with varying concentrations of flucloxacillin, clindamycin or linezolid and PVL expression measured by qRT-PCR and Western blotting. A murine MSSA abscess model was developed to measure leucocidin expression in vivo following antibiotic treatment. RESULTS: 9% (27/314) of MSSA isolates from patients with uncomplicated community skin/soft tissue infections were positive for PVL genes (lukFS-PV). PVL expression by MSSA in broth was unaffected by varying concentrations of flucloxacillin, clindamycin or linezolid. In a murine abscess model, treatment with flucloxacillin did, however, enhance in vivo MSSA lukF-PV transcription and this was sustained even when flucloxacillin was combined with clindamycin, or clindamycin plus linezolid. Notwithstanding increased leucocidin transcription, functional leucotoxin activity was not enhanced. Treatment with flucloxacillin plus clindamycin significantly decreased leucotoxin activity, but the addition of a second protein synthesis inhibitor, linezolid, did not confer benefit. CONCLUSIONS: Our results suggest flucloxacillin in combination with a single protein-synthesis inhibitor such as clindamycin would give the best treatment outcome.
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spelling pubmed-45353172015-09-01 Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics Turner, Claire E. Sriskandan, Shiranee J Infect Article OBJECTIVES: We set out to investigate the impact of common antibiotics on Panton–Valentine Leucocidin (PVL) expression by methicillin-sensitive Staphylococcus aureus (MSSA). PVL expression by methicillin-resistant S. aureus (MRSA) is reportedly enhanced by β-lactams, but inhibited by protein-synthesis inhibitors, a fact that has influenced management of infections associated with PVL. Although PVL is more frequently associated with MSSA than MRSA in the UK, the effect of antibiotics on PVL expression by MSSA has not been fully addressed. METHODS: MSSA was cultured in vitro with varying concentrations of flucloxacillin, clindamycin or linezolid and PVL expression measured by qRT-PCR and Western blotting. A murine MSSA abscess model was developed to measure leucocidin expression in vivo following antibiotic treatment. RESULTS: 9% (27/314) of MSSA isolates from patients with uncomplicated community skin/soft tissue infections were positive for PVL genes (lukFS-PV). PVL expression by MSSA in broth was unaffected by varying concentrations of flucloxacillin, clindamycin or linezolid. In a murine abscess model, treatment with flucloxacillin did, however, enhance in vivo MSSA lukF-PV transcription and this was sustained even when flucloxacillin was combined with clindamycin, or clindamycin plus linezolid. Notwithstanding increased leucocidin transcription, functional leucotoxin activity was not enhanced. Treatment with flucloxacillin plus clindamycin significantly decreased leucotoxin activity, but the addition of a second protein synthesis inhibitor, linezolid, did not confer benefit. CONCLUSIONS: Our results suggest flucloxacillin in combination with a single protein-synthesis inhibitor such as clindamycin would give the best treatment outcome. W.B. Saunders 2015-09 /pmc/articles/PMC4535317/ /pubmed/26028260 http://dx.doi.org/10.1016/j.jinf.2015.05.008 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turner, Claire E.
Sriskandan, Shiranee
Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics
title Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics
title_full Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics
title_fullStr Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics
title_full_unstemmed Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics
title_short Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics
title_sort panton–valentine leucocidin expression by staphylococcus aureus exposed to common antibiotics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535317/
https://www.ncbi.nlm.nih.gov/pubmed/26028260
http://dx.doi.org/10.1016/j.jinf.2015.05.008
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