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The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer

INTRODUCTION: Mesenchymal stromal cells (MSC) are an integral cellular component of the tumor microenvironment. Nevertheless, very little is known about MSC originating from human malignant tissue and modulation of these cells by tumor-derived factors. The aim of this study was to isolate and charac...

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Autores principales: Kansy, Benjamin A, Dißmann, Philip A, Hemeda, Hatim, Bruderek, Kirsten, Westerkamp, Anna M, Jagalski, Vivien, Schuler, Patrick, Kansy, Katinka, Lang, Stephan, Dumitru, Claudia A, Brandau, Sven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535379/
https://www.ncbi.nlm.nih.gov/pubmed/25115189
http://dx.doi.org/10.1186/scrt484
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author Kansy, Benjamin A
Dißmann, Philip A
Hemeda, Hatim
Bruderek, Kirsten
Westerkamp, Anna M
Jagalski, Vivien
Schuler, Patrick
Kansy, Katinka
Lang, Stephan
Dumitru, Claudia A
Brandau, Sven
author_facet Kansy, Benjamin A
Dißmann, Philip A
Hemeda, Hatim
Bruderek, Kirsten
Westerkamp, Anna M
Jagalski, Vivien
Schuler, Patrick
Kansy, Katinka
Lang, Stephan
Dumitru, Claudia A
Brandau, Sven
author_sort Kansy, Benjamin A
collection PubMed
description INTRODUCTION: Mesenchymal stromal cells (MSC) are an integral cellular component of the tumor microenvironment. Nevertheless, very little is known about MSC originating from human malignant tissue and modulation of these cells by tumor-derived factors. The aim of this study was to isolate and characterize MSC from head and neck squamous cell carcinoma (HNSCC) and to investigate their interaction with tumor cells. METHODS: MSC were isolated from tumor tissues of HNSCC patients during routine oncological surgery. Immunophenotyping, immunofluorescence and in vitro differentiation were performed to determine whether the isolated cells met the consensus criteria for MSC. The cytokine profile of tumor-derived MSC was determined by enzyme-linked immunosorbent assay (ELISA). Activation of MSC by tumor-conditioned media was assessed by measuring cytokine release and expression of CD54. The impact of MSC on tumor growth in vivo was analyzed in a HNSCC xenograft model. RESULTS: Cells isolated from HNSCC tissue met the consensus criteria for MSC. Tumor-derived MSC constitutively produced high amounts of interleukin (IL)-6, IL-8 and stromal cell-derived factor (SDF)-1α. HNSCC-derived factors activated MSC and enhanced secretion of IL-8 and expression of CD54. Furthermore, MSC provided stromal support for human HNSCC cell lines in vivo and enhanced their growth in a murine xenograft model. CONCLUSIONS: This is the first study to isolate and characterize MSC from malignant tissues of patients with HNSCC. We observed cross-talk of stromal cells and tumor cells resulting in enhanced growth of HNSCC in vivo.
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spelling pubmed-45353792015-08-14 The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer Kansy, Benjamin A Dißmann, Philip A Hemeda, Hatim Bruderek, Kirsten Westerkamp, Anna M Jagalski, Vivien Schuler, Patrick Kansy, Katinka Lang, Stephan Dumitru, Claudia A Brandau, Sven Stem Cell Res Ther Research INTRODUCTION: Mesenchymal stromal cells (MSC) are an integral cellular component of the tumor microenvironment. Nevertheless, very little is known about MSC originating from human malignant tissue and modulation of these cells by tumor-derived factors. The aim of this study was to isolate and characterize MSC from head and neck squamous cell carcinoma (HNSCC) and to investigate their interaction with tumor cells. METHODS: MSC were isolated from tumor tissues of HNSCC patients during routine oncological surgery. Immunophenotyping, immunofluorescence and in vitro differentiation were performed to determine whether the isolated cells met the consensus criteria for MSC. The cytokine profile of tumor-derived MSC was determined by enzyme-linked immunosorbent assay (ELISA). Activation of MSC by tumor-conditioned media was assessed by measuring cytokine release and expression of CD54. The impact of MSC on tumor growth in vivo was analyzed in a HNSCC xenograft model. RESULTS: Cells isolated from HNSCC tissue met the consensus criteria for MSC. Tumor-derived MSC constitutively produced high amounts of interleukin (IL)-6, IL-8 and stromal cell-derived factor (SDF)-1α. HNSCC-derived factors activated MSC and enhanced secretion of IL-8 and expression of CD54. Furthermore, MSC provided stromal support for human HNSCC cell lines in vivo and enhanced their growth in a murine xenograft model. CONCLUSIONS: This is the first study to isolate and characterize MSC from malignant tissues of patients with HNSCC. We observed cross-talk of stromal cells and tumor cells resulting in enhanced growth of HNSCC in vivo. BioMed Central 2014-08-12 /pmc/articles/PMC4535379/ /pubmed/25115189 http://dx.doi.org/10.1186/scrt484 Text en © Kansy et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kansy, Benjamin A
Dißmann, Philip A
Hemeda, Hatim
Bruderek, Kirsten
Westerkamp, Anna M
Jagalski, Vivien
Schuler, Patrick
Kansy, Katinka
Lang, Stephan
Dumitru, Claudia A
Brandau, Sven
The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
title The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
title_full The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
title_fullStr The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
title_full_unstemmed The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
title_short The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
title_sort bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535379/
https://www.ncbi.nlm.nih.gov/pubmed/25115189
http://dx.doi.org/10.1186/scrt484
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