Cargando…

Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress

Empoasca onukii Matsuda is a worldwide pest that causes great economic loss in tea growing areas and is significantly affected by temperatures. Heat shock protein (Hsp) genes are important in insects’ response to temperature stress. In this study, two full-length Hsp genes, Eohsp90 and Eohsp70, were...

Descripción completa

Detalles Bibliográficos
Autores principales: Qiao, Li, Wu, Jun X., Qin, Dao Z., Liu, Xiang C., Lu, Zhao C., Lv, Li Z., Pan, Zi L., Chen, Hao, Li, Guang W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535498/
https://www.ncbi.nlm.nih.gov/pubmed/25888707
http://dx.doi.org/10.1093/jisesa/iev030
_version_ 1782385612334039040
author Qiao, Li
Wu, Jun X.
Qin, Dao Z.
Liu, Xiang C.
Lu, Zhao C.
Lv, Li Z.
Pan, Zi L.
Chen, Hao
Li, Guang W.
author_facet Qiao, Li
Wu, Jun X.
Qin, Dao Z.
Liu, Xiang C.
Lu, Zhao C.
Lv, Li Z.
Pan, Zi L.
Chen, Hao
Li, Guang W.
author_sort Qiao, Li
collection PubMed
description Empoasca onukii Matsuda is a worldwide pest that causes great economic loss in tea growing areas and is significantly affected by temperatures. Heat shock protein (Hsp) genes are important in insects’ response to temperature stress. In this study, two full-length Hsp genes, Eohsp90 and Eohsp70, were cloned from E. onukii using rapid amplification of complementary DNA ends. The open reading frames of Eohsp90 and Eohsp70 were 2,172 bp and 2,016 bp in length, respectively. Their deduced amino acid sequences of Eohsp90 and Eohsp70 showed high homology with other species. Subsequently, the transcriptional expression of Eohsp90 and Eohsp70 in E. onukii adults exposed to various temperatures (−5, 0, 10, 15, 20, 25, 30, 35, 38, 41 and 44°C) for 1 h, and at extreme temperatures (0°C and 41°C) for various time duration (0, 20, 40, 60, 80, 100, and 120 min) were investigated via real-time quantitative polymerase chain reaction. The relative expression levels of both Eohsp90 and Eohsp70 in E. onukii adults were upregulated as the temperature rises or falls over time, except in the −5°C or 44°C temperature groups. Moreover, the expression level in the temperature elevated groups was higher than that of the lower temperature groups. In addition, the Eohsp70 generally demonstrated a higher transcriptional level than Eohsp90, and both genes had a higher expression profile in female adults compared with the males. The expression profiles indicated that Eohsp90 and Eohsp70 may play important roles in E. onukii adult responses to ecologically relevant environmental temperature threat.
format Online
Article
Text
id pubmed-4535498
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-45354982015-08-17 Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress Qiao, Li Wu, Jun X. Qin, Dao Z. Liu, Xiang C. Lu, Zhao C. Lv, Li Z. Pan, Zi L. Chen, Hao Li, Guang W. J Insect Sci Research Empoasca onukii Matsuda is a worldwide pest that causes great economic loss in tea growing areas and is significantly affected by temperatures. Heat shock protein (Hsp) genes are important in insects’ response to temperature stress. In this study, two full-length Hsp genes, Eohsp90 and Eohsp70, were cloned from E. onukii using rapid amplification of complementary DNA ends. The open reading frames of Eohsp90 and Eohsp70 were 2,172 bp and 2,016 bp in length, respectively. Their deduced amino acid sequences of Eohsp90 and Eohsp70 showed high homology with other species. Subsequently, the transcriptional expression of Eohsp90 and Eohsp70 in E. onukii adults exposed to various temperatures (−5, 0, 10, 15, 20, 25, 30, 35, 38, 41 and 44°C) for 1 h, and at extreme temperatures (0°C and 41°C) for various time duration (0, 20, 40, 60, 80, 100, and 120 min) were investigated via real-time quantitative polymerase chain reaction. The relative expression levels of both Eohsp90 and Eohsp70 in E. onukii adults were upregulated as the temperature rises or falls over time, except in the −5°C or 44°C temperature groups. Moreover, the expression level in the temperature elevated groups was higher than that of the lower temperature groups. In addition, the Eohsp70 generally demonstrated a higher transcriptional level than Eohsp90, and both genes had a higher expression profile in female adults compared with the males. The expression profiles indicated that Eohsp90 and Eohsp70 may play important roles in E. onukii adult responses to ecologically relevant environmental temperature threat. Oxford University Press 2015-04-17 /pmc/articles/PMC4535498/ /pubmed/25888707 http://dx.doi.org/10.1093/jisesa/iev030 Text en © Crown copyright 2015.
spellingShingle Research
Qiao, Li
Wu, Jun X.
Qin, Dao Z.
Liu, Xiang C.
Lu, Zhao C.
Lv, Li Z.
Pan, Zi L.
Chen, Hao
Li, Guang W.
Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress
title Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress
title_full Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress
title_fullStr Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress
title_full_unstemmed Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress
title_short Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress
title_sort gene expression profiles of heat shock proteins 70 and 90 from empoasca onukii (hemiptera: cicadellidae) in response to temperature stress
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535498/
https://www.ncbi.nlm.nih.gov/pubmed/25888707
http://dx.doi.org/10.1093/jisesa/iev030
work_keys_str_mv AT qiaoli geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT wujunx geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT qindaoz geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT liuxiangc geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT luzhaoc geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT lvliz geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT panzil geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT chenhao geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress
AT liguangw geneexpressionprofilesofheatshockproteins70and90fromempoascaonukiihemipteracicadellidaeinresponsetotemperaturestress