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An integrated functional genomic analysis identifies the antitumorigenic mechanism of action for PPARγ in lung cancer cells

Integrating the analysis of the cistrome of a transcription factor by ChIP-Seq with the study of its transcriptional output by microarray or RNA-Seq analysis is a powerful approach to elucidate the genomic functions of a transcription factor. Recently, we employed this approach to determine the mech...

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Detalles Bibliográficos
Autores principales: Kollipara, Rahul K., Kittler, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535999/
https://www.ncbi.nlm.nih.gov/pubmed/26484153
http://dx.doi.org/10.1016/j.gdata.2014.11.015
Descripción
Sumario:Integrating the analysis of the cistrome of a transcription factor by ChIP-Seq with the study of its transcriptional output by microarray or RNA-Seq analysis is a powerful approach to elucidate the genomic functions of a transcription factor. Recently, we employed this approach to determine the mechanism of action by which the nuclear receptor PPARγ elicits its antitumorigenic effects in lung cancer cells upon activation by TZDs (1). Here we describe in detail the design, contents and quality controls for the gene expression and cistrome analyses associated with our study published in Cell Metabolism in 2014.