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Simple Experimental Methods for Determining the Apparent Focal Shift in a Microscope System
Three-dimensional optical microscopy is often complicated by a refractive index mismatch between the sample and objective lens. This mismatch causes focal shift, a difference between sample motion and focal-plane motion, that hinders the accuracy of 3D reconstructions. We present two methods for mea...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536040/ https://www.ncbi.nlm.nih.gov/pubmed/26270960 http://dx.doi.org/10.1371/journal.pone.0134616 |