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A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation
We present a human gut metaproteomic dataset deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001573. Ten aliquots of a single stool sample collected from a healthy human volunteer were either pretreated by differential centrifugation (DC; N...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536287/ https://www.ncbi.nlm.nih.gov/pubmed/26306325 http://dx.doi.org/10.1016/j.dib.2015.07.014 |
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author | Tanca, Alessandro Palomba, Antonio Pisanu, Salvatore Addis, Maria Filippa Uzzau, Sergio |
author_facet | Tanca, Alessandro Palomba, Antonio Pisanu, Salvatore Addis, Maria Filippa Uzzau, Sergio |
author_sort | Tanca, Alessandro |
collection | PubMed |
description | We present a human gut metaproteomic dataset deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001573. Ten aliquots of a single stool sample collected from a healthy human volunteer were either pretreated by differential centrifugation (DC; N=5) or not centrifuged (NC; N=5). Protein extracts were then processed by filter-aided sample preparation, single-run liquid chromatography and high-resolution mass spectrometry, and peptide identification was carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform. The dataset described here is also related to the research article entitled “Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota” published in Proteomics (Tanca et al., 2015), [1]. |
format | Online Article Text |
id | pubmed-4536287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-45362872015-08-24 A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation Tanca, Alessandro Palomba, Antonio Pisanu, Salvatore Addis, Maria Filippa Uzzau, Sergio Data Brief Data Article We present a human gut metaproteomic dataset deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001573. Ten aliquots of a single stool sample collected from a healthy human volunteer were either pretreated by differential centrifugation (DC; N=5) or not centrifuged (NC; N=5). Protein extracts were then processed by filter-aided sample preparation, single-run liquid chromatography and high-resolution mass spectrometry, and peptide identification was carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform. The dataset described here is also related to the research article entitled “Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota” published in Proteomics (Tanca et al., 2015), [1]. Elsevier 2015-07-26 /pmc/articles/PMC4536287/ /pubmed/26306325 http://dx.doi.org/10.1016/j.dib.2015.07.014 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Tanca, Alessandro Palomba, Antonio Pisanu, Salvatore Addis, Maria Filippa Uzzau, Sergio A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation |
title | A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation |
title_full | A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation |
title_fullStr | A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation |
title_full_unstemmed | A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation |
title_short | A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation |
title_sort | human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536287/ https://www.ncbi.nlm.nih.gov/pubmed/26306325 http://dx.doi.org/10.1016/j.dib.2015.07.014 |
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