High-yield isolation of extracellular vesicles using aqueous two-phase system

Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have...

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Detalles Bibliográficos
Autores principales: Shin, Hyunwoo, Han, Chungmin, Labuz, Joseph M., Kim, Jiyoon, Kim, Jongmin, Cho, Siwoo, Gho, Yong Song, Takayama, Shuichi, Park, Jaesung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536486/
https://www.ncbi.nlm.nih.gov/pubmed/26271727
http://dx.doi.org/10.1038/srep13103
Descripción
Sumario:Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.

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