High-yield isolation of extracellular vesicles using aqueous two-phase system

Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have...

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Autores principales: Shin, Hyunwoo, Han, Chungmin, Labuz, Joseph M., Kim, Jiyoon, Kim, Jongmin, Cho, Siwoo, Gho, Yong Song, Takayama, Shuichi, Park, Jaesung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536486/
https://www.ncbi.nlm.nih.gov/pubmed/26271727
http://dx.doi.org/10.1038/srep13103
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author Shin, Hyunwoo
Han, Chungmin
Labuz, Joseph M.
Kim, Jiyoon
Kim, Jongmin
Cho, Siwoo
Gho, Yong Song
Takayama, Shuichi
Park, Jaesung
author_facet Shin, Hyunwoo
Han, Chungmin
Labuz, Joseph M.
Kim, Jiyoon
Kim, Jongmin
Cho, Siwoo
Gho, Yong Song
Takayama, Shuichi
Park, Jaesung
author_sort Shin, Hyunwoo
collection PubMed
description Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.
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spelling pubmed-45364862015-09-04 High-yield isolation of extracellular vesicles using aqueous two-phase system Shin, Hyunwoo Han, Chungmin Labuz, Joseph M. Kim, Jiyoon Kim, Jongmin Cho, Siwoo Gho, Yong Song Takayama, Shuichi Park, Jaesung Sci Rep Article Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs. Nature Publishing Group 2015-08-14 /pmc/articles/PMC4536486/ /pubmed/26271727 http://dx.doi.org/10.1038/srep13103 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Shin, Hyunwoo
Han, Chungmin
Labuz, Joseph M.
Kim, Jiyoon
Kim, Jongmin
Cho, Siwoo
Gho, Yong Song
Takayama, Shuichi
Park, Jaesung
High-yield isolation of extracellular vesicles using aqueous two-phase system
title High-yield isolation of extracellular vesicles using aqueous two-phase system
title_full High-yield isolation of extracellular vesicles using aqueous two-phase system
title_fullStr High-yield isolation of extracellular vesicles using aqueous two-phase system
title_full_unstemmed High-yield isolation of extracellular vesicles using aqueous two-phase system
title_short High-yield isolation of extracellular vesicles using aqueous two-phase system
title_sort high-yield isolation of extracellular vesicles using aqueous two-phase system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536486/
https://www.ncbi.nlm.nih.gov/pubmed/26271727
http://dx.doi.org/10.1038/srep13103
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