TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton

Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using...

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Detalles Bibliográficos
Autores principales: Preston Campbell, J., Mulcrone, P., Masood, S. K., Karolak, M., Merkel, A., Hebron, K., Zijlstra, A., Sterling, J., Elefteriou, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536516/
https://www.ncbi.nlm.nih.gov/pubmed/26271202
http://dx.doi.org/10.1038/srep12635
Descripción
Sumario:Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.

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