TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton

Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using...

Descripción completa

Detalles Bibliográficos
Autores principales: Preston Campbell, J., Mulcrone, P., Masood, S. K., Karolak, M., Merkel, A., Hebron, K., Zijlstra, A., Sterling, J., Elefteriou, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536516/
https://www.ncbi.nlm.nih.gov/pubmed/26271202
http://dx.doi.org/10.1038/srep12635
_version_ 1782385754980220928
author Preston Campbell, J.
Mulcrone, P.
Masood, S. K.
Karolak, M.
Merkel, A.
Hebron, K.
Zijlstra, A.
Sterling, J.
Elefteriou, F.
author_facet Preston Campbell, J.
Mulcrone, P.
Masood, S. K.
Karolak, M.
Merkel, A.
Hebron, K.
Zijlstra, A.
Sterling, J.
Elefteriou, F.
author_sort Preston Campbell, J.
collection PubMed
description Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.
format Online
Article
Text
id pubmed-4536516
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-45365162015-09-04 TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton Preston Campbell, J. Mulcrone, P. Masood, S. K. Karolak, M. Merkel, A. Hebron, K. Zijlstra, A. Sterling, J. Elefteriou, F. Sci Rep Article Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models. Nature Publishing Group 2015-08-14 /pmc/articles/PMC4536516/ /pubmed/26271202 http://dx.doi.org/10.1038/srep12635 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Preston Campbell, J.
Mulcrone, P.
Masood, S. K.
Karolak, M.
Merkel, A.
Hebron, K.
Zijlstra, A.
Sterling, J.
Elefteriou, F.
TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
title TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
title_full TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
title_fullStr TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
title_full_unstemmed TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
title_short TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
title_sort trizol and alu qpcr-based quantification of metastatic seeding within the skeleton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536516/
https://www.ncbi.nlm.nih.gov/pubmed/26271202
http://dx.doi.org/10.1038/srep12635
work_keys_str_mv AT prestoncampbellj trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT mulcronep trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT masoodsk trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT karolakm trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT merkela trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT hebronk trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT zijlstraa trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT sterlingj trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton
AT elefteriouf trizolandaluqpcrbasedquantificationofmetastaticseedingwithintheskeleton

Ejemplares similares