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TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using...
Autores principales: | Preston Campbell, J., Mulcrone, P., Masood, S. K., Karolak, M., Merkel, A., Hebron, K., Zijlstra, A., Sterling, J., Elefteriou, F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536516/ https://www.ncbi.nlm.nih.gov/pubmed/26271202 http://dx.doi.org/10.1038/srep12635 |
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