Cargando…
Cerebrospinal Fluid Biomarkers of Japanese Encephalitis
Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infe...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000Research
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536617/ https://www.ncbi.nlm.nih.gov/pubmed/26309732 http://dx.doi.org/10.12688/f1000research.6801.1 |
_version_ | 1782385767130071040 |
---|---|
author | Sengupta, Nabonita Mukherjee, Sriparna Tripathi, Piyush Kumar, Rashmi Suryavanshi, Amol Basu, Anirban |
author_facet | Sengupta, Nabonita Mukherjee, Sriparna Tripathi, Piyush Kumar, Rashmi Suryavanshi, Amol Basu, Anirban |
author_sort | Sengupta, Nabonita |
collection | PubMed |
description | Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost. The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker. |
format | Online Article Text |
id | pubmed-4536617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | F1000Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-45366172015-08-24 Cerebrospinal Fluid Biomarkers of Japanese Encephalitis Sengupta, Nabonita Mukherjee, Sriparna Tripathi, Piyush Kumar, Rashmi Suryavanshi, Amol Basu, Anirban F1000Res Research Article Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost. The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker. F1000Research 2015-07-23 /pmc/articles/PMC4536617/ /pubmed/26309732 http://dx.doi.org/10.12688/f1000research.6801.1 Text en Copyright: © 2015 Sengupta N et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/publicdomain/zero/1.0/ Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication). |
spellingShingle | Research Article Sengupta, Nabonita Mukherjee, Sriparna Tripathi, Piyush Kumar, Rashmi Suryavanshi, Amol Basu, Anirban Cerebrospinal Fluid Biomarkers of Japanese Encephalitis |
title | Cerebrospinal Fluid Biomarkers of Japanese Encephalitis |
title_full | Cerebrospinal Fluid Biomarkers of Japanese Encephalitis |
title_fullStr | Cerebrospinal Fluid Biomarkers of Japanese Encephalitis |
title_full_unstemmed | Cerebrospinal Fluid Biomarkers of Japanese Encephalitis |
title_short | Cerebrospinal Fluid Biomarkers of Japanese Encephalitis |
title_sort | cerebrospinal fluid biomarkers of japanese encephalitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536617/ https://www.ncbi.nlm.nih.gov/pubmed/26309732 http://dx.doi.org/10.12688/f1000research.6801.1 |
work_keys_str_mv | AT senguptanabonita cerebrospinalfluidbiomarkersofjapaneseencephalitis AT mukherjeesriparna cerebrospinalfluidbiomarkersofjapaneseencephalitis AT tripathipiyush cerebrospinalfluidbiomarkersofjapaneseencephalitis AT kumarrashmi cerebrospinalfluidbiomarkersofjapaneseencephalitis AT suryavanshiamol cerebrospinalfluidbiomarkersofjapaneseencephalitis AT basuanirban cerebrospinalfluidbiomarkersofjapaneseencephalitis |