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Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity

BACKGROUND: Clinical and preclinical observations indicate that Lactobacillus plantarum has anti-inflammatory activity and may regulate the immune responses of its hosts when ingested. Recently, modification of teichoic acids (TAs) produced by L. plantarum was reported as a key to regulating the sys...

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Autores principales: Liu, Wei-Hsien, Yang, Chih-Hsien, Lin, Ching-Ting, Li, Shiao-Wen, Cheng, Wei-Shen, Jiang, Yi-Ping, Wu, Chien-Chen, Chang, Chuan-Hsiung, Tsai, Ying-Chieh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536865/
https://www.ncbi.nlm.nih.gov/pubmed/26279684
http://dx.doi.org/10.1186/s13099-015-0068-y
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author Liu, Wei-Hsien
Yang, Chih-Hsien
Lin, Ching-Ting
Li, Shiao-Wen
Cheng, Wei-Shen
Jiang, Yi-Ping
Wu, Chien-Chen
Chang, Chuan-Hsiung
Tsai, Ying-Chieh
author_facet Liu, Wei-Hsien
Yang, Chih-Hsien
Lin, Ching-Ting
Li, Shiao-Wen
Cheng, Wei-Shen
Jiang, Yi-Ping
Wu, Chien-Chen
Chang, Chuan-Hsiung
Tsai, Ying-Chieh
author_sort Liu, Wei-Hsien
collection PubMed
description BACKGROUND: Clinical and preclinical observations indicate that Lactobacillus plantarum has anti-inflammatory activity and may regulate the immune responses of its hosts when ingested. Recently, modification of teichoic acids (TAs) produced by L. plantarum was reported as a key to regulating the systemic immune response in mice. However, data linking TA-related genetic determinants and the immunomodulatory effect are limited. To provide genomic information for elucidating the underlying mechanism of immunomodulation by L. plantarum, we sequenced the genome of L. plantarum strain PS128. RESULTS: The PS128 genome contains 11 contigs (3,325,806 bp; 44.42% GC content) after hybrid assembly of sequences derived with Illumina MiSeq and PacBio RSII systems. The most abundant functions of the protein-coding genes are carbohydrate, amino acid, and protein metabolism. The 16S rDNA sequences of PS128 are closest to the sequences of L. plantarum WCFS1 and B21; these three strains form a distinct clade based on 16S rDNA sequences. PS128 shares core genes encoding the metabolism, transport, and modification of TAs with other sequenced L. plantarum strains. Compared with the TA-related genes of other completely sequenced L. plantarum strains, the PS128 contains more lipoteichoic acid exporter genes. CONCLUSIONS: We determined the draft genome sequence of PS128 and compared its TA-related genes with those of other L. plantarum strains. Shared genomic features with respect to TA-related subsystems may be important clues to the mechanism by which L. plantarum regulates its host immune responses, but unique TA-related genetic determinants should be further investigated to elucidate strain-specific immunomodulatory effects. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13099-015-0068-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-45368652015-08-15 Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity Liu, Wei-Hsien Yang, Chih-Hsien Lin, Ching-Ting Li, Shiao-Wen Cheng, Wei-Shen Jiang, Yi-Ping Wu, Chien-Chen Chang, Chuan-Hsiung Tsai, Ying-Chieh Gut Pathog Short Report BACKGROUND: Clinical and preclinical observations indicate that Lactobacillus plantarum has anti-inflammatory activity and may regulate the immune responses of its hosts when ingested. Recently, modification of teichoic acids (TAs) produced by L. plantarum was reported as a key to regulating the systemic immune response in mice. However, data linking TA-related genetic determinants and the immunomodulatory effect are limited. To provide genomic information for elucidating the underlying mechanism of immunomodulation by L. plantarum, we sequenced the genome of L. plantarum strain PS128. RESULTS: The PS128 genome contains 11 contigs (3,325,806 bp; 44.42% GC content) after hybrid assembly of sequences derived with Illumina MiSeq and PacBio RSII systems. The most abundant functions of the protein-coding genes are carbohydrate, amino acid, and protein metabolism. The 16S rDNA sequences of PS128 are closest to the sequences of L. plantarum WCFS1 and B21; these three strains form a distinct clade based on 16S rDNA sequences. PS128 shares core genes encoding the metabolism, transport, and modification of TAs with other sequenced L. plantarum strains. Compared with the TA-related genes of other completely sequenced L. plantarum strains, the PS128 contains more lipoteichoic acid exporter genes. CONCLUSIONS: We determined the draft genome sequence of PS128 and compared its TA-related genes with those of other L. plantarum strains. Shared genomic features with respect to TA-related subsystems may be important clues to the mechanism by which L. plantarum regulates its host immune responses, but unique TA-related genetic determinants should be further investigated to elucidate strain-specific immunomodulatory effects. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13099-015-0068-y) contains supplementary material, which is available to authorized users. BioMed Central 2015-08-15 /pmc/articles/PMC4536865/ /pubmed/26279684 http://dx.doi.org/10.1186/s13099-015-0068-y Text en © Liu et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Liu, Wei-Hsien
Yang, Chih-Hsien
Lin, Ching-Ting
Li, Shiao-Wen
Cheng, Wei-Shen
Jiang, Yi-Ping
Wu, Chien-Chen
Chang, Chuan-Hsiung
Tsai, Ying-Chieh
Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity
title Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity
title_full Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity
title_fullStr Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity
title_full_unstemmed Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity
title_short Genome architecture of Lactobacillus plantarum PS128, a probiotic strain with potential immunomodulatory activity
title_sort genome architecture of lactobacillus plantarum ps128, a probiotic strain with potential immunomodulatory activity
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536865/
https://www.ncbi.nlm.nih.gov/pubmed/26279684
http://dx.doi.org/10.1186/s13099-015-0068-y
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