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Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation

BACKGROUND: Biofilm formation is a major virulence factor in different bacteria. Biofilms allow bacteria to resist treatment with antibacterial agents. The biofilm formation on glass and steel surfaces, which are extremely useful surfaces in food industries and medical devices, has always had an imp...

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Autores principales: Ghasemian, Ehsan, Naghoni, Ali, Rahvar, Helya, Kialha, Mahsa, Tabaraie, Bahman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4537522/
https://www.ncbi.nlm.nih.gov/pubmed/26290685
http://dx.doi.org/10.5812/jjm.8(5)2015.17430
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author Ghasemian, Ehsan
Naghoni, Ali
Rahvar, Helya
Kialha, Mahsa
Tabaraie, Bahman
author_facet Ghasemian, Ehsan
Naghoni, Ali
Rahvar, Helya
Kialha, Mahsa
Tabaraie, Bahman
author_sort Ghasemian, Ehsan
collection PubMed
description BACKGROUND: Biofilm formation is a major virulence factor in different bacteria. Biofilms allow bacteria to resist treatment with antibacterial agents. The biofilm formation on glass and steel surfaces, which are extremely useful surfaces in food industries and medical devices, has always had an important role in the distribution and transmission of infectious diseases. OBJECTIVES: In this study, the effect of coating glass and steel surfaces by copper nanoparticles (CuNPs) in inhibiting the biofilm formation by Listeria monocytogenes and Pseudomonas aeruginosa was examined. MATERIALS AND METHODS: The minimal inhibitory concentrations (MICs) of synthesized CuNPs were measured against L. monocytogenes and P. aeruginosa by using the broth-dilution method. The cell-surface hydrophobicity of the selected bacteria was assessed using the bacterial adhesion to hydrocarbon (BATH) method. Also, the effect of the CuNP-coated surfaces on the biofilm formation of the selected bacteria was calculated via the surface assay. RESULTS: The MICs for the CuNPs according to the broth-dilution method were ≤ 16 mg/L for L. monocytogenes and ≤ 32 mg/L for P. aeruginosa. The hydrophobicity of P. aeruginosa and L. monocytogenes was calculated as 74% and 67%, respectively. The results for the surface assay showed a significant decrease in bacterial attachment and colonization on the CuNP-covered surfaces. CONCLUSIONS: Our data demonstrated that the CuNPs inhibited bacterial growth and that the CuNP-coated surfaces decreased the microbial count and the microbial biofilm formation. Such CuNP-coated surfaces can be used in medical devices and food industries, although further studies in order to measure their level of toxicity would be necessary.
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spelling pubmed-45375222015-08-19 Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation Ghasemian, Ehsan Naghoni, Ali Rahvar, Helya Kialha, Mahsa Tabaraie, Bahman Jundishapur J Microbiol Research Article BACKGROUND: Biofilm formation is a major virulence factor in different bacteria. Biofilms allow bacteria to resist treatment with antibacterial agents. The biofilm formation on glass and steel surfaces, which are extremely useful surfaces in food industries and medical devices, has always had an important role in the distribution and transmission of infectious diseases. OBJECTIVES: In this study, the effect of coating glass and steel surfaces by copper nanoparticles (CuNPs) in inhibiting the biofilm formation by Listeria monocytogenes and Pseudomonas aeruginosa was examined. MATERIALS AND METHODS: The minimal inhibitory concentrations (MICs) of synthesized CuNPs were measured against L. monocytogenes and P. aeruginosa by using the broth-dilution method. The cell-surface hydrophobicity of the selected bacteria was assessed using the bacterial adhesion to hydrocarbon (BATH) method. Also, the effect of the CuNP-coated surfaces on the biofilm formation of the selected bacteria was calculated via the surface assay. RESULTS: The MICs for the CuNPs according to the broth-dilution method were ≤ 16 mg/L for L. monocytogenes and ≤ 32 mg/L for P. aeruginosa. The hydrophobicity of P. aeruginosa and L. monocytogenes was calculated as 74% and 67%, respectively. The results for the surface assay showed a significant decrease in bacterial attachment and colonization on the CuNP-covered surfaces. CONCLUSIONS: Our data demonstrated that the CuNPs inhibited bacterial growth and that the CuNP-coated surfaces decreased the microbial count and the microbial biofilm formation. Such CuNP-coated surfaces can be used in medical devices and food industries, although further studies in order to measure their level of toxicity would be necessary. Kowsar 2015-05-31 /pmc/articles/PMC4537522/ /pubmed/26290685 http://dx.doi.org/10.5812/jjm.8(5)2015.17430 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Ghasemian, Ehsan
Naghoni, Ali
Rahvar, Helya
Kialha, Mahsa
Tabaraie, Bahman
Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation
title Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation
title_full Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation
title_fullStr Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation
title_full_unstemmed Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation
title_short Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation
title_sort evaluating the effect of copper nanoparticles in inhibiting pseudomonas aeruginosa and listeria monocytogenes biofilm formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4537522/
https://www.ncbi.nlm.nih.gov/pubmed/26290685
http://dx.doi.org/10.5812/jjm.8(5)2015.17430
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