Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

BACKGROUND: Distinguishing between viable and dead bacteria in animal and urban effluents is a major challenge. Among existing methods, propidium monoazide (PMA)-qPCR is a promising way to quantify viable cells. However, its efficiency depends on the composition of the effluent, particularly on tota...

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Detalles Bibliográficos
Autores principales: Desneux, Jérémy, Chemaly, Marianne, Pourcher, Anne-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4537567/
https://www.ncbi.nlm.nih.gov/pubmed/26276157
http://dx.doi.org/10.1186/s12866-015-0505-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4537567/
https://www.ncbi.nlm.nih.gov/pubmed/26276157
http://dx.doi.org/10.1186/s12866-015-0505-6

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