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Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents
BACKGROUND: Distinguishing between viable and dead bacteria in animal and urban effluents is a major challenge. Among existing methods, propidium monoazide (PMA)-qPCR is a promising way to quantify viable cells. However, its efficiency depends on the composition of the effluent, particularly on tota...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4537567/ https://www.ncbi.nlm.nih.gov/pubmed/26276157 http://dx.doi.org/10.1186/s12866-015-0505-6 |