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Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts

Replicative senescence is of fundamental importance for the process of cellular aging, since it is a property of most of our somatic cells. Here, we elucidated this process by comparing gene expression changes, measured by RNA-seq, in fibroblasts originating from two different tissues, embryonic lun...

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Autores principales: Marthandan, Shiva, Priebe, Steffen, Baumgart, Mario, Groth, Marco, Cellerino, Alessandro, Guthke, Reinhard, Hemmerich, Peter, Diekmann, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538583/
https://www.ncbi.nlm.nih.gov/pubmed/26339636
http://dx.doi.org/10.1155/2015/731938
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author Marthandan, Shiva
Priebe, Steffen
Baumgart, Mario
Groth, Marco
Cellerino, Alessandro
Guthke, Reinhard
Hemmerich, Peter
Diekmann, Stephan
author_facet Marthandan, Shiva
Priebe, Steffen
Baumgart, Mario
Groth, Marco
Cellerino, Alessandro
Guthke, Reinhard
Hemmerich, Peter
Diekmann, Stephan
author_sort Marthandan, Shiva
collection PubMed
description Replicative senescence is of fundamental importance for the process of cellular aging, since it is a property of most of our somatic cells. Here, we elucidated this process by comparing gene expression changes, measured by RNA-seq, in fibroblasts originating from two different tissues, embryonic lung (MRC-5) and foreskin (HFF), at five different time points during their transition into senescence. Although the expression patterns of both fibroblast cell lines can be clearly distinguished, the similar differential expression of an ensemble of genes was found to correlate well with their transition into senescence, with only a minority of genes being cell line specific. Clustering-based approaches further revealed common signatures between the cell lines. Investigation of the mRNA expression levels at various time points during the lifespan of either of the fibroblasts resulted in a number of monotonically up- and downregulated genes which clearly showed a novel strong link to aging and senescence related processes which might be functional. In terms of expression profiles of differentially expressed genes with age, common genes identified here have the potential to rule the transition into senescence of embryonic lung and foreskin fibroblasts irrespective of their different cellular origin.
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spelling pubmed-45385832015-09-03 Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts Marthandan, Shiva Priebe, Steffen Baumgart, Mario Groth, Marco Cellerino, Alessandro Guthke, Reinhard Hemmerich, Peter Diekmann, Stephan Biomed Res Int Research Article Replicative senescence is of fundamental importance for the process of cellular aging, since it is a property of most of our somatic cells. Here, we elucidated this process by comparing gene expression changes, measured by RNA-seq, in fibroblasts originating from two different tissues, embryonic lung (MRC-5) and foreskin (HFF), at five different time points during their transition into senescence. Although the expression patterns of both fibroblast cell lines can be clearly distinguished, the similar differential expression of an ensemble of genes was found to correlate well with their transition into senescence, with only a minority of genes being cell line specific. Clustering-based approaches further revealed common signatures between the cell lines. Investigation of the mRNA expression levels at various time points during the lifespan of either of the fibroblasts resulted in a number of monotonically up- and downregulated genes which clearly showed a novel strong link to aging and senescence related processes which might be functional. In terms of expression profiles of differentially expressed genes with age, common genes identified here have the potential to rule the transition into senescence of embryonic lung and foreskin fibroblasts irrespective of their different cellular origin. Hindawi Publishing Corporation 2015 2015-08-03 /pmc/articles/PMC4538583/ /pubmed/26339636 http://dx.doi.org/10.1155/2015/731938 Text en Copyright © 2015 Shiva Marthandan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Marthandan, Shiva
Priebe, Steffen
Baumgart, Mario
Groth, Marco
Cellerino, Alessandro
Guthke, Reinhard
Hemmerich, Peter
Diekmann, Stephan
Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
title Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
title_full Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
title_fullStr Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
title_full_unstemmed Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
title_short Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
title_sort similarities in gene expression profiles during in vitro aging of primary human embryonic lung and foreskin fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538583/
https://www.ncbi.nlm.nih.gov/pubmed/26339636
http://dx.doi.org/10.1155/2015/731938
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