The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments

We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent...

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Detalles Bibliográficos
Autores principales: Rodríguez, Jéssica, Mosquera, Jesús, Couceiro, Jose R., Vázquez, M. Eugenio, Mascareñas, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538796/
https://www.ncbi.nlm.nih.gov/pubmed/26290687
http://dx.doi.org/10.1039/c5sc01415h
Descripción
Sumario:We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the conjugates.

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