The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments

We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodríguez, Jéssica, Mosquera, Jesús, Couceiro, Jose R., Vázquez, M. Eugenio, Mascareñas, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538796/
https://www.ncbi.nlm.nih.gov/pubmed/26290687
http://dx.doi.org/10.1039/c5sc01415h
_version_ 1782386034944770048
author Rodríguez, Jéssica
Mosquera, Jesús
Couceiro, Jose R.
Vázquez, M. Eugenio
Mascareñas, José L.
author_facet Rodríguez, Jéssica
Mosquera, Jesús
Couceiro, Jose R.
Vázquez, M. Eugenio
Mascareñas, José L.
author_sort Rodríguez, Jéssica
collection PubMed
description We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the conjugates.
format Online
Article
Text
id pubmed-4538796
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-45387962015-08-17 The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments Rodríguez, Jéssica Mosquera, Jesús Couceiro, Jose R. Vázquez, M. Eugenio Mascareñas, José L. Chem Sci Chemistry We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the conjugates. Royal Society of Chemistry 2015-08-01 2015-05-26 /pmc/articles/PMC4538796/ /pubmed/26290687 http://dx.doi.org/10.1039/c5sc01415h Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Rodríguez, Jéssica
Mosquera, Jesús
Couceiro, Jose R.
Vázquez, M. Eugenio
Mascareñas, José L.
The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments
title The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments
title_full The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments
title_fullStr The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments
title_full_unstemmed The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments
title_short The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments
title_sort at-hook motif as a versatile minor groove anchor for promoting dna binding of transcription factor fragments
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538796/
https://www.ncbi.nlm.nih.gov/pubmed/26290687
http://dx.doi.org/10.1039/c5sc01415h
work_keys_str_mv AT rodriguezjessica theathookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT mosquerajesus theathookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT couceirojoser theathookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT vazquezmeugenio theathookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT mascarenasjosel theathookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT rodriguezjessica athookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT mosquerajesus athookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT couceirojoser athookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT vazquezmeugenio athookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments
AT mascarenasjosel athookmotifasaversatileminorgrooveanchorforpromotingdnabindingoftranscriptionfactorfragments

Ejemplares similares