Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another en...

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Autores principales: Vuoristo, Kiira S., Mars, Astrid E., van Loon, Stijn, Orsi, Enrico, Eggink, Gerrit, Sanders, Johan P. M., Weusthuis, Ruud A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4539527/
https://www.ncbi.nlm.nih.gov/pubmed/26347730
http://dx.doi.org/10.3389/fmicb.2015.00849
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author Vuoristo, Kiira S.
Mars, Astrid E.
van Loon, Stijn
Orsi, Enrico
Eggink, Gerrit
Sanders, Johan P. M.
Weusthuis, Ruud A.
author_facet Vuoristo, Kiira S.
Mars, Astrid E.
van Loon, Stijn
Orsi, Enrico
Eggink, Gerrit
Sanders, Johan P. M.
Weusthuis, Ruud A.
author_sort Vuoristo, Kiira S.
collection PubMed
description Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.
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spelling pubmed-45395272015-09-07 Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production Vuoristo, Kiira S. Mars, Astrid E. van Loon, Stijn Orsi, Enrico Eggink, Gerrit Sanders, Johan P. M. Weusthuis, Ruud A. Front Microbiol Microbiology Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L. Frontiers Media S.A. 2015-08-18 /pmc/articles/PMC4539527/ /pubmed/26347730 http://dx.doi.org/10.3389/fmicb.2015.00849 Text en Copyright © 2015 Vuoristo, Mars, van Loon, Orsi, Eggink, Sanders and Weusthuis. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Vuoristo, Kiira S.
Mars, Astrid E.
van Loon, Stijn
Orsi, Enrico
Eggink, Gerrit
Sanders, Johan P. M.
Weusthuis, Ruud A.
Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production
title Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production
title_full Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production
title_fullStr Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production
title_full_unstemmed Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production
title_short Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production
title_sort heterologous expression of mus musculus immunoresponsive gene 1 (irg1) in escherichia coli results in itaconate production
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4539527/
https://www.ncbi.nlm.nih.gov/pubmed/26347730
http://dx.doi.org/10.3389/fmicb.2015.00849
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