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Development and validation of a simple, sensitive enzyme immunoassay for quantification of androstenedione in bull plasma

As an alternative to radioimmunoassay a simple and highly sensitive enzyme immunoassay (EIA) was developed and validated for androstenedione quantification in plasma of Karan Fries bulls using second antibody coating technique. The wells of the microtitreplate were coated with affinity-purified goat...

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Detalles Bibliográficos
Autores principales: Mallick, Smrutirekha, Kumar, BS Bharath, Prakash, BS, Aggrawal, Anjali, Pandita, Sujata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540262/
https://www.ncbi.nlm.nih.gov/pubmed/26290733
http://dx.doi.org/10.1186/s40781-014-0035-z