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Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)

The fat mass and obesity associated (FTO) gene plays an important role in the regulation of energy homeostasis, fat deposition and obesity. For this reason, the FTO gene is a physiological and functional candidate gene for carcass and meat quality traits in beef cattle. The objectives of this study...

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Autor principal: Chung, Eui-Ryong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540293/
https://www.ncbi.nlm.nih.gov/pubmed/26290716
http://dx.doi.org/10.1186/2055-0391-56-27
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author Chung, Eui-Ryong
author_facet Chung, Eui-Ryong
author_sort Chung, Eui-Ryong
collection PubMed
description The fat mass and obesity associated (FTO) gene plays an important role in the regulation of energy homeostasis, fat deposition and obesity. For this reason, the FTO gene is a physiological and functional candidate gene for carcass and meat quality traits in beef cattle. The objectives of this study were to identify SNPs in the exonic regions of FTO gene and to evaluate the association of these SNPs with carcass traits in Hanwoo (Korean cattle). In this study, we newly identified two exonic SNPs in Hanwoo population. The g.125550A > T SNP was located in exon 3 and the g.175675C > T SNP was located in exon 6. Genotyping of the two SNP markers was carried out using PCR-RFLP analysis in Hanwoo steers to evaluate their association with carcass traits. As a result, g.125550A > T SNP genotype was significantly associated with effects on marbling score. Animals with the AA and TT homozygous genotypes had a significantly higher marbling score (p < 0.001) than those with AT heterozygous genotype, and this was significant after Bonferroni correction of the significance threshold (p = 0.003). Dominance effect was also observed for the marbling score (P < 0.05) with higher marbling score of homozygous animals. However, no significant associations with meat quality traits were observed for the g.175675C > T SNP. Our results suggest that the exonic SNP g.125550A > T in the FTO gene may be used as a DNA marker for the selection of Hanwoo with higher marbling.
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spelling pubmed-45402932015-08-19 Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle) Chung, Eui-Ryong J Anim Sci Technol Research The fat mass and obesity associated (FTO) gene plays an important role in the regulation of energy homeostasis, fat deposition and obesity. For this reason, the FTO gene is a physiological and functional candidate gene for carcass and meat quality traits in beef cattle. The objectives of this study were to identify SNPs in the exonic regions of FTO gene and to evaluate the association of these SNPs with carcass traits in Hanwoo (Korean cattle). In this study, we newly identified two exonic SNPs in Hanwoo population. The g.125550A > T SNP was located in exon 3 and the g.175675C > T SNP was located in exon 6. Genotyping of the two SNP markers was carried out using PCR-RFLP analysis in Hanwoo steers to evaluate their association with carcass traits. As a result, g.125550A > T SNP genotype was significantly associated with effects on marbling score. Animals with the AA and TT homozygous genotypes had a significantly higher marbling score (p < 0.001) than those with AT heterozygous genotype, and this was significant after Bonferroni correction of the significance threshold (p = 0.003). Dominance effect was also observed for the marbling score (P < 0.05) with higher marbling score of homozygous animals. However, no significant associations with meat quality traits were observed for the g.175675C > T SNP. Our results suggest that the exonic SNP g.125550A > T in the FTO gene may be used as a DNA marker for the selection of Hanwoo with higher marbling. BioMed Central 2014-12-03 /pmc/articles/PMC4540293/ /pubmed/26290716 http://dx.doi.org/10.1186/2055-0391-56-27 Text en © Chung; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Chung, Eui-Ryong
Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)
title Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)
title_full Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)
title_fullStr Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)
title_full_unstemmed Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)
title_short Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)
title_sort novel snp in the coding region of the fto gene is associated with marbling score in hanwoo (korean cattle)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540293/
https://www.ncbi.nlm.nih.gov/pubmed/26290716
http://dx.doi.org/10.1186/2055-0391-56-27
work_keys_str_mv AT chungeuiryong novelsnpinthecodingregionoftheftogeneisassociatedwithmarblingscoreinhanwookoreancattle