Molecular cloning and characterization of Izumo1 gene from bovine testis

A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expressio...

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Autor principal: Kim, Ekyune
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540309/
https://www.ncbi.nlm.nih.gov/pubmed/26290736
http://dx.doi.org/10.1186/s40781-015-0049-1
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author Kim, Ekyune
author_facet Kim, Ekyune
author_sort Kim, Ekyune
collection PubMed
description A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expression analysis of bovine IZUMO1 (bIZUMO1). RT-PCR and Western blot analysis of the bovine tissues indicated that bIZUMO1 was specifically expressed in the testis and sperm, Furthermore, the result of our biotinylation assay from ejaculated bovine sperm strongly suggest the assumption that bIZUMO1 is localized on the cell surface. These data imply the potential role of bovine IZUMO1 in mammalian fertilization.
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spelling pubmed-45403092015-08-19 Molecular cloning and characterization of Izumo1 gene from bovine testis Kim, Ekyune J Anim Sci Technol Short Report A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expression analysis of bovine IZUMO1 (bIZUMO1). RT-PCR and Western blot analysis of the bovine tissues indicated that bIZUMO1 was specifically expressed in the testis and sperm, Furthermore, the result of our biotinylation assay from ejaculated bovine sperm strongly suggest the assumption that bIZUMO1 is localized on the cell surface. These data imply the potential role of bovine IZUMO1 in mammalian fertilization. BioMed Central 2015-04-09 /pmc/articles/PMC4540309/ /pubmed/26290736 http://dx.doi.org/10.1186/s40781-015-0049-1 Text en © Kim; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Kim, Ekyune
Molecular cloning and characterization of Izumo1 gene from bovine testis
title Molecular cloning and characterization of Izumo1 gene from bovine testis
title_full Molecular cloning and characterization of Izumo1 gene from bovine testis
title_fullStr Molecular cloning and characterization of Izumo1 gene from bovine testis
title_full_unstemmed Molecular cloning and characterization of Izumo1 gene from bovine testis
title_short Molecular cloning and characterization of Izumo1 gene from bovine testis
title_sort molecular cloning and characterization of izumo1 gene from bovine testis
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540309/
https://www.ncbi.nlm.nih.gov/pubmed/26290736
http://dx.doi.org/10.1186/s40781-015-0049-1
work_keys_str_mv AT kimekyune molecularcloningandcharacterizationofizumo1genefrombovinetestis

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