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Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain

With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were network...

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Autores principales: Lee, Ho-Sung, Kang, Dai-In, Yoon, Seung Zhoo, Ryu, Yeon Hee, Lee, Inhyung, Kim, Hoon-Gi, Lee, Byung-Cheon, Lee, Ki Bog
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541241/
https://www.ncbi.nlm.nih.gov/pubmed/26330833
http://dx.doi.org/10.4103/1673-5374.160103
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author Lee, Ho-Sung
Kang, Dai-In
Yoon, Seung Zhoo
Ryu, Yeon Hee
Lee, Inhyung
Kim, Hoon-Gi
Lee, Byung-Cheon
Lee, Ki Bog
author_facet Lee, Ho-Sung
Kang, Dai-In
Yoon, Seung Zhoo
Ryu, Yeon Hee
Lee, Inhyung
Kim, Hoon-Gi
Lee, Byung-Cheon
Lee, Ki Bog
author_sort Lee, Ho-Sung
collection PubMed
description With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.
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spelling pubmed-45412412015-09-01 Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain Lee, Ho-Sung Kang, Dai-In Yoon, Seung Zhoo Ryu, Yeon Hee Lee, Inhyung Kim, Hoon-Gi Lee, Byung-Cheon Lee, Ki Bog Neural Regen Res Research Article With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix. Medknow Publications & Media Pvt Ltd 2015-07 /pmc/articles/PMC4541241/ /pubmed/26330833 http://dx.doi.org/10.4103/1673-5374.160103 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Ho-Sung
Kang, Dai-In
Yoon, Seung Zhoo
Ryu, Yeon Hee
Lee, Inhyung
Kim, Hoon-Gi
Lee, Byung-Cheon
Lee, Ki Bog
Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
title Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
title_full Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
title_fullStr Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
title_full_unstemmed Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
title_short Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
title_sort evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541241/
https://www.ncbi.nlm.nih.gov/pubmed/26330833
http://dx.doi.org/10.4103/1673-5374.160103
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