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Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain
With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were network...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541241/ https://www.ncbi.nlm.nih.gov/pubmed/26330833 http://dx.doi.org/10.4103/1673-5374.160103 |
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author | Lee, Ho-Sung Kang, Dai-In Yoon, Seung Zhoo Ryu, Yeon Hee Lee, Inhyung Kim, Hoon-Gi Lee, Byung-Cheon Lee, Ki Bog |
author_facet | Lee, Ho-Sung Kang, Dai-In Yoon, Seung Zhoo Ryu, Yeon Hee Lee, Inhyung Kim, Hoon-Gi Lee, Byung-Cheon Lee, Ki Bog |
author_sort | Lee, Ho-Sung |
collection | PubMed |
description | With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix. |
format | Online Article Text |
id | pubmed-4541241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45412412015-09-01 Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain Lee, Ho-Sung Kang, Dai-In Yoon, Seung Zhoo Ryu, Yeon Hee Lee, Inhyung Kim, Hoon-Gi Lee, Byung-Cheon Lee, Ki Bog Neural Regen Res Research Article With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix. Medknow Publications & Media Pvt Ltd 2015-07 /pmc/articles/PMC4541241/ /pubmed/26330833 http://dx.doi.org/10.4103/1673-5374.160103 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lee, Ho-Sung Kang, Dai-In Yoon, Seung Zhoo Ryu, Yeon Hee Lee, Inhyung Kim, Hoon-Gi Lee, Byung-Cheon Lee, Ki Bog Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain |
title | Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain |
title_full | Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain |
title_fullStr | Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain |
title_full_unstemmed | Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain |
title_short | Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain |
title_sort | evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541241/ https://www.ncbi.nlm.nih.gov/pubmed/26330833 http://dx.doi.org/10.4103/1673-5374.160103 |
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