Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes

In recent years, obesity has been considered a pathological stage of early lifestyle-related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingol...

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Autores principales: Kojima, Hisao, Suzuki, Yusuke, Ito, Masahiro, Kabayama, Kazuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541715/
https://www.ncbi.nlm.nih.gov/pubmed/26017029
http://dx.doi.org/10.1007/s11745-015-4035-7
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author Kojima, Hisao
Suzuki, Yusuke
Ito, Masahiro
Kabayama, Kazuya
author_facet Kojima, Hisao
Suzuki, Yusuke
Ito, Masahiro
Kabayama, Kazuya
author_sort Kojima, Hisao
collection PubMed
description In recent years, obesity has been considered a pathological stage of early lifestyle-related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingolipid molecular species composition of adipocytes have yet to be elucidated. We used 3T3-L1 adipocytes and the 1,2-dichloroethane-wash method to remove triacylglycerols, which are abundant in adipocytes, and analyzed the structures of glycosphingolipids, particularly neutral glycosphingolipids, using liquid chromatography–mass spectrometry. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11745-015-4035-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-45417152015-08-21 Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes Kojima, Hisao Suzuki, Yusuke Ito, Masahiro Kabayama, Kazuya Lipids Communication In recent years, obesity has been considered a pathological stage of early lifestyle-related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingolipid molecular species composition of adipocytes have yet to be elucidated. We used 3T3-L1 adipocytes and the 1,2-dichloroethane-wash method to remove triacylglycerols, which are abundant in adipocytes, and analyzed the structures of glycosphingolipids, particularly neutral glycosphingolipids, using liquid chromatography–mass spectrometry. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11745-015-4035-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-05-28 2015 /pmc/articles/PMC4541715/ /pubmed/26017029 http://dx.doi.org/10.1007/s11745-015-4035-7 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Communication
Kojima, Hisao
Suzuki, Yusuke
Ito, Masahiro
Kabayama, Kazuya
Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes
title Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes
title_full Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes
title_fullStr Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes
title_full_unstemmed Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes
title_short Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes
title_sort structural characterization of neutral glycosphingolipids from 3t3-l1 adipocytes
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541715/
https://www.ncbi.nlm.nih.gov/pubmed/26017029
http://dx.doi.org/10.1007/s11745-015-4035-7
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