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Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?

Gene flow and hybridization among species dramatically affect our understanding of the species as a biological unit, species relationships, and species adaptations. In North American Colias eurytheme and Colias eriphyle, there has been historical debate over the extent of hybridization occurring and...

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Autores principales: Dwyer, Heather E, Jasieniuk, Marie, Okada, Miki, Shapiro, Arthur M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541991/
https://www.ncbi.nlm.nih.gov/pubmed/26306172
http://dx.doi.org/10.1002/ece3.1574
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author Dwyer, Heather E
Jasieniuk, Marie
Okada, Miki
Shapiro, Arthur M
author_facet Dwyer, Heather E
Jasieniuk, Marie
Okada, Miki
Shapiro, Arthur M
author_sort Dwyer, Heather E
collection PubMed
description Gene flow and hybridization among species dramatically affect our understanding of the species as a biological unit, species relationships, and species adaptations. In North American Colias eurytheme and Colias eriphyle, there has been historical debate over the extent of hybridization occurring and the identity of phenotypically intermediate individuals as genetic hybrids. This study assesses the population structure of these two species to measure the extent of hybridization and the genetic identity of phenotypic intermediates as hybrids. Amplified fragment length polymorphism (AFLP) marker analysis was performed on 378 specimens collected from northern California and Nevada. Population structure was inferred using a Bayesian/Markov chain Monte Carlo method, which probabilistically assigns individuals to genetic clusters. Three genetic clusters provided the best fit for the data. C. eurytheme individuals were primarily assigned to two closely related clusters, and C. eriphyle individuals were mostly assigned to a third, more distantly related cluster. There appeared to be significant hybridization between the two species. Individuals of intermediate phenotype (putative hybrids) were found to be genetically indistinguishable from C. eriphyle, indicating that previous work based on the assumption that these intermediate forms are hybrids may warrant reconsideration.
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spelling pubmed-45419912015-08-24 Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids? Dwyer, Heather E Jasieniuk, Marie Okada, Miki Shapiro, Arthur M Ecol Evol Original Research Gene flow and hybridization among species dramatically affect our understanding of the species as a biological unit, species relationships, and species adaptations. In North American Colias eurytheme and Colias eriphyle, there has been historical debate over the extent of hybridization occurring and the identity of phenotypically intermediate individuals as genetic hybrids. This study assesses the population structure of these two species to measure the extent of hybridization and the genetic identity of phenotypic intermediates as hybrids. Amplified fragment length polymorphism (AFLP) marker analysis was performed on 378 specimens collected from northern California and Nevada. Population structure was inferred using a Bayesian/Markov chain Monte Carlo method, which probabilistically assigns individuals to genetic clusters. Three genetic clusters provided the best fit for the data. C. eurytheme individuals were primarily assigned to two closely related clusters, and C. eriphyle individuals were mostly assigned to a third, more distantly related cluster. There appeared to be significant hybridization between the two species. Individuals of intermediate phenotype (putative hybrids) were found to be genetically indistinguishable from C. eriphyle, indicating that previous work based on the assumption that these intermediate forms are hybrids may warrant reconsideration. John Wiley & Sons, Ltd 2015-07 2015-06-25 /pmc/articles/PMC4541991/ /pubmed/26306172 http://dx.doi.org/10.1002/ece3.1574 Text en © 2015 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Dwyer, Heather E
Jasieniuk, Marie
Okada, Miki
Shapiro, Arthur M
Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
title Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
title_full Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
title_fullStr Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
title_full_unstemmed Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
title_short Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
title_sort molecular evidence for hybridization in colias (lepidoptera: pieridae): are colias hybrids really hybrids?
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4541991/
https://www.ncbi.nlm.nih.gov/pubmed/26306172
http://dx.doi.org/10.1002/ece3.1574
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