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Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere
Anaerobic oxidation of ammonium (anammox) is recognized as an important process for nitrogen (N) cycling, yet its role in agricultural ecosystems, which are intensively fertilized, remains unclear. In this study, we investigated the presence, activity, functional gene abundance and role of anammox b...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542037/ https://www.ncbi.nlm.nih.gov/pubmed/25689022 http://dx.doi.org/10.1038/ismej.2015.25 |
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author | Nie, San'an Li, Hu Yang, Xiaoru Zhang, Zhaoji Weng, Bosen Huang, Fuyi Zhu, Gui-Bing Zhu, Yong-Guan |
author_facet | Nie, San'an Li, Hu Yang, Xiaoru Zhang, Zhaoji Weng, Bosen Huang, Fuyi Zhu, Gui-Bing Zhu, Yong-Guan |
author_sort | Nie, San'an |
collection | PubMed |
description | Anaerobic oxidation of ammonium (anammox) is recognized as an important process for nitrogen (N) cycling, yet its role in agricultural ecosystems, which are intensively fertilized, remains unclear. In this study, we investigated the presence, activity, functional gene abundance and role of anammox bacteria in rhizosphere and non-rhizosphere paddy soils using catalyzed reporter deposition–fluorescence in situ hybridization, isotope-tracing technique, quantitative PCR assay and 16S rRNA gene clone libraries. Results showed that rhizosphere anammox contributed to 31–41% N(2) production with activities of 0.33–0.64 nmol N(2) g(−1) soil h(−1), whereas the non-rhizosphere anammox bacteria contributed to only 2–3% N(2) production with lower activities of 0.08–0.26 nmol N(2) g(−1) soil h(−1). Higher anammox bacterial cells were observed (0.75–1.4 × 10(7) copies g(−1) soil) in the rhizosphere, which were twofold higher compared with the non-rhizosphere soil (3.7–5.9 × 10(6) copies g(−1) soil). Phylogenetic analysis of the anammox bacterial 16S rRNA genes indicated that two genera of ‘Candidatus Kuenenia' and ‘Candidatus Brocadia' and the family of Planctomycetaceae were identified. We suggest the rhizosphere provides a favorable niche for anammox bacteria, which are important to N cycling, but were previously largely overlooked. |
format | Online Article Text |
id | pubmed-4542037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45420372015-09-01 Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere Nie, San'an Li, Hu Yang, Xiaoru Zhang, Zhaoji Weng, Bosen Huang, Fuyi Zhu, Gui-Bing Zhu, Yong-Guan ISME J Original Article Anaerobic oxidation of ammonium (anammox) is recognized as an important process for nitrogen (N) cycling, yet its role in agricultural ecosystems, which are intensively fertilized, remains unclear. In this study, we investigated the presence, activity, functional gene abundance and role of anammox bacteria in rhizosphere and non-rhizosphere paddy soils using catalyzed reporter deposition–fluorescence in situ hybridization, isotope-tracing technique, quantitative PCR assay and 16S rRNA gene clone libraries. Results showed that rhizosphere anammox contributed to 31–41% N(2) production with activities of 0.33–0.64 nmol N(2) g(−1) soil h(−1), whereas the non-rhizosphere anammox bacteria contributed to only 2–3% N(2) production with lower activities of 0.08–0.26 nmol N(2) g(−1) soil h(−1). Higher anammox bacterial cells were observed (0.75–1.4 × 10(7) copies g(−1) soil) in the rhizosphere, which were twofold higher compared with the non-rhizosphere soil (3.7–5.9 × 10(6) copies g(−1) soil). Phylogenetic analysis of the anammox bacterial 16S rRNA genes indicated that two genera of ‘Candidatus Kuenenia' and ‘Candidatus Brocadia' and the family of Planctomycetaceae were identified. We suggest the rhizosphere provides a favorable niche for anammox bacteria, which are important to N cycling, but were previously largely overlooked. Nature Publishing Group 2015-09 2015-02-17 /pmc/articles/PMC4542037/ /pubmed/25689022 http://dx.doi.org/10.1038/ismej.2015.25 Text en Copyright © 2015 International Society for Microbial Ecology http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Article Nie, San'an Li, Hu Yang, Xiaoru Zhang, Zhaoji Weng, Bosen Huang, Fuyi Zhu, Gui-Bing Zhu, Yong-Guan Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere |
title | Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere |
title_full | Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere |
title_fullStr | Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere |
title_full_unstemmed | Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere |
title_short | Nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere |
title_sort | nitrogen loss by anaerobic oxidation of ammonium in rice rhizosphere |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542037/ https://www.ncbi.nlm.nih.gov/pubmed/25689022 http://dx.doi.org/10.1038/ismej.2015.25 |
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